NORGEN BIOTEK ^CORP. Product Information Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit Norgen's Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Kits provides a fast, reliable reproducible and simple procedure for isolating total circulating Nucleic Acid (DNA and RNA) from various amounts of plasma/serum inputs ranging from 50 |jl_ up to 5 mL, with various kit formats address different plasma/serum input volumes. Purification is based on spin column chromatography that uses Norgen's proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-DNA and circulating RNA, including microRNA, as well as all sizes of exosomal RNA. Norgen's Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Kits provide a clear advantage over other avail- able kits in that they do not require phenol/chloroform or any protease treatments. Nucleic Acids can be isolated from either fresh or frozen samples using this kit. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 25 jL to 100 jL No phenol:chloroform Circulating DNA/RNA and Exosomal RNA are isolated without the extractions use of harmful chemicals such as phenol or chloroform. Isolate all sizes of circulating The kit allows for the isolation of all sizes of fragmented circulating RNA and exosomal RNA RNA and exosomal RNA, including microRNA . Fast and easy processing Input volume Rapid spin column format allows for the processing of multiple samples in under 35-40 minutes. Isolate circulating nucleic acid from 250 jL to 1.5 jL of plasma/ serum. Purified NA can be used in a number of sensitive downstream applications including reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. © Norgen Biotek Corp. 2015

Product Information Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit Figure 1. Purification of cell-free circulating DNA from different plasma volumes. Norgen’s Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit (Cat# 56400) was used to purify circulating DNA from 250 µL, 750 µL and 1.5 mL plasma prepared from blood collected on citrate as an anticoagulant. Two microlitres of the purified NA was then used as the template in qPCR reactions to assess the relative...

Product Information Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit Figure 4. Purification of cell-free circulating RNA and Exosomal RNA from different plasma volumes. Norgen’s Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit (Cat# 56400) was used to purify cell-free circulating and exosomal RNA from 250 µL, 750 µL and 1.5 mL plasma prepared from blood collected on citrate as an anticoagulant in comparison. Two microlitres of the purified RNA was then...

Product Information Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit Figure 6. Determination of the amount of inhibition present in plasma RNA samples when detecting the human 5S transcript and mir-21. RNA was isolated from 250 µL, 750 µL and 1.5 mL plasma using Norgen’s Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit (Cat# 56400). Increasing volumes of the elution (2, 4 and 8 μL) were used in a 20 μL reverse transcription reaction followed by qPCR amplification...