Product Information RNA/DNA/Protein Purification Plus Kit NORGEN BIOTEK ^CORP. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing silicon carbide columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The pro- teins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. Complete column The RNA, DNA and proteins are all column purified using the same purification column. Reduce variability RNA, DNA and proteins are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variability. Isolate from small samples Sequential isolation of RNA, DNA and protein from a single sample. Ideal for precious, difficult to obtain or small samples such as biopsy material or single foci from cell cultures. Rapid procedure Isolate total RNA, genomic DNA and total proteins from a single sample in < 30 minutes. Isolate a diversity of RNA All sizes of RNA are isolated, from large mRNA down to microRNA species Process a wide range of Isolate total RNA, genomic DNA and proteins from cultured animal sample types cells , tissue, blood, bacteria, yeast, fungi and plants. © Norgen Biotek Corp. 2015

Product Information RNA/DNA/Protein Purification Plus Kit RNA/DNA/Protein Purification Plus Kit Figure 1. Recovery of True Total RNA including microRNA from HEK-293 Cells. Panel A is a 1X MOPS 1% agarose gel showing the RNA that was isolated from 2 different samples of ~ 800,000 HEK-293 cells using either Norgen's RNA/DNA/Protein Purification Plus Kit or a competitor's multiple-analyte purification kit. Both kits isolated large RNA (represented by 28S and 18S rRNA) with high integrity but Norgen's RNA/DNA/Protein Purification Plus Kit provided the added benefit of recovering small RNA without...

Product Information RNA/DNA/Protein Purification Plus Kit RNA/DNA/Protein Purification Plus Kit Figure 2. Recovery of True Total RNA including microRNA from Hamster Liver. Panel A is a 1X MOPS 1% agarose gel showing the RNA that was isolated from 2 different samples of 15 mg hamster liver using either Norgen's RNA/DNA/Protein Purification Plus Kit or a competitor's multiple-analyte purification kit. Both kits isolated large RNA (represented by 28S and 18S rRNA) with high integrity but Norgen's RNA/DNA/Protein Purification Plus Kit provided the added benefit of recovering small RNA without any...