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Total RNA Purification Plus Kit

Total RNA Purification Plus Kit
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Total RNA Purification Plus Kit

Product catalog summary
Product Overview: The document describes the Total RNA Purification Plus Kit by Norgen Biotek Corp., designed for the extraction of high-quality and pure total RNA, including miRNA, from samples without the need for a phenol step. The kit efficiently removes genomic DNA (gDNA) using a specialized removal column.
Specifications: The kit is available in two sizes, 50 preps (Cat. #48300) and 100 preps (Cat. #48400), with a micro kit option (Cat. #48500). It features a maximum binding capacity of 50 μg and a loading volume per spin column of 650 μL. The kit can purify RNA of all sizes, including those less than 200 nucleotides, and complete 10 purifications in 20 minutes.
Performance: The RNA yield is specified as 15 μg for 1 x 106 HeLa cells and 50 μg for 1 x 109 E. coli cells. The kit is noted for its sensitivity and linearity, capable of processing down to a few cells without the need for carrier RNA.
Quality Standards: Norgen Biotek Corp. is registered under ISO 13485:2003, ISO 9001:2008, and ISO 15189:2012, ensuring compliance with international quality standards.
Contact Information: For further inquiries, Norgen Biotek Corp. can be contacted via phone at 1-866-NORGENB or 905-227-8848, fax at 905-227-1061, or email at [email protected]. More information is available on their website, www.norgenbiotek.com.
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Catalog excerpts

Total RNA Purification Plus Kit-1

Total RNA Purification Plus Kit NORGEN CORP. BIOTEK Product Features Extract high quality & purity total RNA including miRNA No phenol step required; isolate all RNA in one fraction Genomic DNA Removal Column for efficient elimination of gDNA Bind & elute all RNA irrespective of size or GC content, without bias Very sensitive & linear down to a few cells without the need for carrier RNA Genomic DNA Removal from HeLa Cells and ΔCt Values Observed Between Different gDNA Removal Techniques A M Removal Column Removal Column gDNA Removal Column Kit Specifications Maximum Binding Capacity Maximum Loading Loading Volume Per Spin Column Size of RNA Purified Time to Complete 10 Purifications RNA Yield HeLa (1 x 106) E. coli (1 x 109) All sizes, including < 200 nt 20 minutes Prep Size Total RNA Purification Plus Kit Total RNA Purification Plus Micro Kit NORGEN CORP. BIOTEK The Sample Preparation Experts

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