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ihcDirect® Cytokeratin 7 Kit

ihcDirect® Cytokeratin 7 Kit

Product catalog summary
Product Overview:
The ihcDirect® Cytokeratin 7 Kit is designed for in vitro diagnostic use to identify the presence of Cytokeratin 7 (CK7) in tissue samples using immunohistochemistry (IHC). It is suitable for both frozen and formalin-fixed, paraffin-embedded (FFPE) tissues. The kit includes a rabbit-derived anti-human CK7 antibody labeled with polymerized horseradish peroxidase (polyHRP).

Specifications:
  • Catalog Number: K42015-XXX
  • Package Sizes: -005 for ~50 tests, -010 for ~100 tests
  • Storage: Store at 4°C, do not freeze

Intended Use:
The kit is intended for laboratory use to qualitatively identify CK7 expression in simple glandular and transitional epithelium. It is crucial for the clinical interpretation of staining results to be complemented by morphological studies and evaluated within the context of the patient's clinical history by a qualified pathologist or physician.

Summary and Explanation:
The CK7 antibody recognizes a 55 kD filament protein within the cytokeratin family. It is expressed in most carcinomas except those originating from the colon, prostate, kidney, thymus, and certain tumors like carcinoid tumors and Merkel cell tumors. The kit includes an ihc Blocker to reduce background staining and uses 3,3’diaminobenzidine (DAB) as a chromogen.

Applications:
The kit is applicable for detecting CK7 in lung adenocarcinoma using frozen tissue and breast cancer using FFPE tissue.
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Catalog excerpts

ihcDirect® Cytokeratin 7 Kit-1

Revolutionizing tissue-based diagnostics ihcDirect® Cytokeratin 7 Kit Anti-Human Cytokeratin 7 (Clone R284) Breast Cancer Using FFPE Tissue Lung Adenocarcinoma Using Frozen Tissue Catalog Number: K42015-XXX: Package Size: (XXX) -005: For ~50 IHC Tests using frozen or FFPE tissues -010: For ~100 IHC Tests using frozen or FFPE tissues Store at 4°C upon receiving. DO NOT FREEZE. ihcDirect Cytokeratin 7 (CK7) is a polymerized horseradish peroxidase (polyHRP) labeled CK7 (R284) antibody intended for laboratory use to qualitatively identify by light microscopy the presence of CK7 which is expressed in simple glandular epithelium, and in transitional epithelium, in sections of formalin-fixed, paraffin-embedded (FFPE) tissue or frozen tissue sections using immunohistochemistry (IHC) test method. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests and proper controls interpreted by a qualified pathologist and/or physician. This conjugate has been pre-diluted and optimized for IHC use without further dilution. Summary and Explanation: This antibody recognizes human cytokeratin 7 and is filament protein of 55 kD molecular weight within the cytokeratin family. ihcDirect Cytokeratin 7 (Clone R284) polyHRP antibody conjugate immunohistochemistry (IHC) reagent is for use on frozen and formalin-fixed-paraffin-embedded tissues. Clone R284 antibody reacts with cytokeratin 7 which is found in simple glandular epithelium, and in transitional epithelium. Cytokeratin 7 is expressed in the majority of cases of carcinoma, with exception of those carcinoma arising from the colon, prostate, kidney, and thymus; carcinoid tumors of the lung and gastrointestinal tract origin; Merkel cell tumor of the skin. The ihc Blocker provided in the kit used prior to applying pHRP Cytokeratin 7 conjugate can reduce background and nonspecific staining. Chromogen 3,3’diaminobenzidine (DAB) is used in this kit. www.novodiax.com [email protected] [email protected] 3517 Breakwater Ave Hayward, CA 94545, USA Phone: +1 (888) 439-2716 EMERGO EUROPE Prinsessegracht 20 2514 AP The Hague The Netherla

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