STUDY OF DISINFECTANT ACTION SANITIZING TOOL FOR AIR MODEL DNA C.A. FRANCO LEONI NUOVA ASAV SNC VIA DELL’INDUSTRIA, 44, 42025 CAVRIAGO (RE) – PIVA E CF: 0074191035

Ali Lab srl Via del Quaresimo, 6 42123 Reggio Emilia – ITALIA Microbiology and Quality manager: Dr.ssa Miriam Fornaciari Ali Lab srl – Via del Quaresimo 6, 42123 Codemondo (RE) – P.IVA 02777690351 Telefono: 0522-372363 – email: info@alil

OBJECTIVE By carrying out the following study, we wanted to test the reduction of microbial and fungi load in the air of a closed environment by a new model of ultraviolet ray device developed by the Nuova ASAV snc company. All tests were carried out in a Microbiology Laboratory. The chosen environment is a place where there is the possibility of contracting infections, a risk mainly due to exposure to a wide range of types of agents, from bacteria to fungi. It was deliberately decided to go and evaluate the action of the machine during a period of work within a very critical environment from...

The use of the SAS provides for the positioning of the Contact petri dishes in the special housing positioned under a perforated cover (disinfected each time with sodium hypochlorite). 180 liters of air were sucked onto each plate and the results obtained from the sampling were brought back to 1 m3. The air samplings were carried out in two different areas of the laboratory: • Point 1: Half room near window side; • Point 2: Near door side. The samplings were always carried out with operators present during their work activities. The first sampling was carried out without the use of the DNA tool...

RESULTS The results of the different air samples are summarized in Table 1, where are reported the UFC / m3 and the percentage reduction related to the microbiological parameters detected in the Laboratory air at the two different sampling points. Table 2 indicates the average of the percentage reduction for the various microbiological parameters considered. Table 1. Microbial contamination levels and % reduction for Points 1 and 2. The percentage reduction varies differently depending on the parameter considered: Colony count at 30°C A reduction of more than 80% is achieved after 60 minutes...

Table 2 . Medium % reduction. The table does not include data regarding the spores of the reducing sulfite Clostridia as they were not detected in the environment as early as the beginning of the sampling, i.e. with the tool off. CONCLUSIONS First, it is interesting to note the initial situation of the Laboratory air. The sampling showed a limited contamination of the environment mainly by Colony count at 30°C, Moulds and Staphylococci. The charges of Coliforms and Yeasts were low, and the Sulfite-reducing bacteria were completely absent. In Table 3 it is possible to see the % reduction relating...

Table 4. Average % reduction trend. Both in Table 3 and in Table 4 it is noted that from 50 minutes onwards the blast chilling power of the machine begins to become predominant, with percentages ≥ 80%. If the starting concentrations proved to be very high (such as molds and total microbial load) the abatement power is greater than 90% after 180 minutes. The results show how DNA, already after 50-60 minutes of ignition, manages to completely break down Staphylococci and Coliforms and begins to work consistently also on Colony count at 30°C and Moulds. The performance of the yeasts is not very...