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Gene Synthesis Gene Cloning Site-directed Mutagenesis Recombinant Protein Production molecular services

OLIGOS Oligo synthesis NZYTech delivers oligos produced following rigorous quality control checks with fast turnaround times. Customers can select from Salt Free, HPSF and HPLC Purifications, from 10 to 1000 nmol scale and the resulting oligos are suitable for most Molecular Biology applications such PCR, qPCR, sequencing, hybridization and antisense studies. Additionally, NZYTech delivers an extensive series of modifications. Please contact us at [email protected] for pricing. DNA Probe synthesis NZYTech offers a comprehensive portfolio of DNA probes that are particularly useful for gene expression,...

GENE SYNTHESIS Gene synthesis has become an important tool in many fields of recombinant DNA technology. Whether you have limited cloning experience or simply want to save time, NZYTech’s Gene Synthesis service helps you to quickly move your ideas from the planning stage to the laboratory. A highly motivated team is eager to provide you with a very efficient service affording considerable savings of time and money. Either if you select the custom or High-Throughput (HTP) Gene Synthesis service you will be offered a premium service that will deliver high quality and downstream efficacy. Requested...

Custom Gene Synthesis NZYTech’s Custom Gene Synthesis service provides in vitro chemical synthesis, cloning and 100% sequence verification of any desired DNA sequence. Genes are cloned into our standard cloning vectors. Downstream services, including sub-cloning, site-directed mutagenesis, plasmid preparation and protein expression are available upon request. Gene Synthesis Gene Synthesis (DNA fragments <500 bp) Gene Synthesis (DNA fragments 501-1000 bp) Gene Synthesis (DNA fragments 1001-2000 bp) Gene Synthesis (DNA fragments > than 5001 bp) Gene Synthesis (DNA fragments 2001-5000 bp) Flexible,...

GENE CLONING Custom Gene Cloning NZYTech will customize your plasmid DNA according with your requests. By using next generation cloning tecnhiques, we will quickly join any insert to a plasmid at any location. NZYTech has a comprehensive library of several E. coli expression plasmids - pHTP expression vectors - containing different tags known to enhance protein’s solubility and/or promote correct protein’s folding. This may constitute the best economic and flexible alternative for selecting an appropriate tag for each type of protein. We offer a high performance DNA cloning service allowing you...

High-Throughput Gene Cloning NZYTech offers a high-throughput cloning service for large projects typically involving several hundreds to thousands of genes. A proprietary method is used for cloning your genes into one or more selected pHTP vectors. Our method does not require the use of conventional restriction enzymes and DNA ligases, which increases speed while improving cloning efficiencies. Only the desired DNA fragments are cloned into the destination vector(s) in the correct orientation. HTP Gene Sub-cloning (> 48 genes) HS00201 into pHTP vectors

SITE-DIRECTED MUTAGENESIS NZYTech offers a site-directed mutagenesis service which includes substitution, deletion or insertion of any base pair into a DNA sequence. The strategy usually involves an oligonucleotide-directed mutagenic approach using the provided DNA template. Site-directed mutagenesis - Standard - 1 mutant Site-directed mutagenesis - Standard - 3 mutants Site-directed mutagenesis - Standard - 5 mutants Site-directed mutagenesis - Complex and/or Multiple 1. Mutated primer design 2. PCR using a proofreading polymerase 3. Parental DNA digestion with DpnI Template plasmid DNA supplied...

PLASMID DNA PREPARATION NZYTech’s plasmid DNA preparation services are amenable to small research labs and to large-scale biotech or pharmaceutical companies. Our high-quality plasmids obtained from Escherichia coli cultures will help you to achieve highly efficient cell transfection, as well as microarray analysis, viral packaging, mammalian cell expression, support DNA vaccine development, antibody production, among others. Plasmid DNA Preparation Miniprep (2-10 μg), <24 preps Plasmid DNA Preparation Miniprep (2-10 μg), 24-48 preps Plasmid DNA Preparation Miniprep (2-10 μg), >48 preps Plasmid...

RECOMBINANT PROTEIN PRODUCTION Standard Protein Production Our dedicated team of scientists has worked to offer you a high-quality and cost-effective recombinant protein expression service. Protein production can be scaled up from one to several liter volumes of Escherichia coli culture. High-quality recombinant proteins are purified through Immobilized Metal Affinity Chromatography (IMAC). Recombinant Protein Production in Escherichia coli MS00401 Expression system: E. coli High-quality recombinant proteins purified through IMAC Proteins are provided from µg to mg scale Complementary services...

PEPTIDES Peptide synthesis NZYTech offers a high-quality peptide synthesis service designed to ensure our customer’s complete satisfaction. Every peptide is subjected to a variety of performance control steps to guarantee the highest quality. Peptides may be provided crude, desalted, or with 75% to 98% purity. In addition, NZYTech peptides can be subjected to a variety of chemical modifications upon request. Lyophilized peptide delivered with the required sequence, purity and quantity Several modifications available: phosphorylation, methylation, acetylation, amidation, fluorescence/dye labeling...