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FVMPE-RS Twin Laser System

FVMPE-RS Twin Laser System
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Multiphoton Laser Scanning Microscope Your Vision, Our Future - Advanced multiphoton microscopy with extended IR range at high speed

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Innovative, precise, HIGH-SPEED, and deeply focused High speed Extended IR range Laser light stimulation Deep observation

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The Olympus FVMPE-RS satisfies a myriad of performance needs for deep observation. It delivers high-speed millisecond imaging essential for the capture of rapid in vivo responses, and offers ideal spot excitation with intense energy up to 1300 nm – even at deep sites. It also offers high S/N imaging for the efficient detection of scattered fluorescence photons, simultaneous dual-wavelength excitation at deep sites, visible or multiphoton laser light stimulation, and synchronisation with patch clamp data. Put simply, the Olympus FLUOVIEW FVMPE-RS combines high-speed, deep observation...

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HIGH-SPEED SCANNING CAPTURES IN VIVO RESPONSES WITH 438 fps A high-speed scanner providing unique 438 fps at 512 × 32 scan performance The scanner unit combines a newly developed high-speed resonant scanner with a conventional galvanometer scanner to provide high-speed and high-definition imaging in a single system. High-speed imaging delivers 30 fps at 512 × 512 at full field of view (FN 18), while clip scans optimise return time to achieve an unmatched 438 fps at 512 × 32 pixels – making it possible to capture rapid calcium channel reactions and membrane potentialsensitive dyes in action....

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A cooled, high-sensitivity GaAsP detector acquires high S/N images High S/N imaging can be acquired even under faint fluorescence through the use of gallium arsenide phosphide (GaAsP) in the photomultiplier tube (PMT) – delivering greater quantum efficiency than multi-alkali PMT along with Peltier cooling that improves S/N even further. Image with multi-alkali detector Image captured with GaAsP detector Arc-dVenus transgenic mouse (8 weeks old), coronal brain block, hippocampal dentate gyrus Projection image of 300–400 μm depth (5 μm steps) Image data courtesy of: Dr Norio Takata, Dr Hajime...

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Optimised for Deep Observation Laser with negative chirp improves excitation efficiency at the focal plane A laser beam with optimally adjusted pulse widths can be delivered to the focal plane, thanks to the application of negative dispersion that perfectly corresponds to the magnitude of the pulse-width dispersion generated during transmission through the microscope optics. The result is brighter images without needing to increase laser power, sample heating, photobleaching or photo-toxicity. General multiphoton microscope Multiphoton microscope with negative chirp Negative chirp Deep...

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Deep Observation 100 Optics with new outperforming IR coating 90 An innovative IR coating (160080coating) for the 25× dedicated 70 multiphoton objective range and scanner optics further refines 60 50 deep observation quality. This 40 coating with improved long 30 wavelength transmittance enables excitation without a decrease 20 10 in laser power, even at deep sites. Since transmittance at 405 nm 0 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 also remains high, the feature is700suited to uncaging applications that employ a 405 nm laser. Wavelength (nm) XLPLN25XWMP2 with...

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High-precision laser beam control up to 1300 nm for flexible dual-line Multiphoton imaging Multi-wavelength excitation and multiphoton imaging Multichannel multiphoton excitation imaging is accomplished with a dual-wavelength IR pulsed laser or two independent IR pulsed lasers – enabling simultaneous excitation of chromophores with different wavelengths. Thanks to the flexible and precise IR introduction optics, both lines are accurately merged. Simultaneous excitation provides perfected registration and balanced images for different chromophores. Optimal excitation wavelengths for...

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Extended IR – Dual-Line Imaging

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TOOLS FOR ADVANCED APPLICATIONS Light-stimulation SIM scanner from the visible to IR range A laser light stimulation scanner can be installed separately to form a unique Triple Scanner system. This enables optogenetics laser light stimulation of Channel Rhodopsin (ChR2) and Halorodopsin (NpHR) with simultaneous real-time imaging of neural cell activity with a visible or IR-range laser. •Wide choice of scan modes The FVMPE-RS comes with AOM as standard and provides fine position and time control of imaging and light stimulation. Using Olympus’ own tornado scanning allows rapid bleaching and...

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Laser Light Stimulation 3D-mapping stimulation creates reaction maps based on multiple coordinates Highly targeted laser light stimulation is achieved by dividing the observation domain into a grid and laser irradiating each specific area in a software-controlled sequence while eliminating adjacent areas from stimulation. The Z-position setting is available to enable stimulation at a depth different from that of the imaging layer. Changes in intensity during stimulation can also be mapped onto the image and reaction maps can be created for multiple coordinates. Fluorescence image A typical...

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A varied LINE-UP offering high flexibility and innovative IR laser beam control Single-laser system This streamlined system uses a single multiphoton IR laser for imaging. A SIM scanner for visible laser light stimulation is optional. System desk Power supply unit and chiller Rack (option) System desk Power supply unit and chiller Rack (option) Power supply unit and chiller Rack (Option) 1650 Rack 2550 System desk System desk Power supply unit and chiller System desk Power supply unit and chiller 2000 Power supply unit 3100 System desk This system employs two multiphoton IR 20 Power Power...

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Lasers adapted for a range of multiphoton configurations InSight DeepSee enables dual-wavelength simultaneous imaging for deep observation – with a high peak power with short 120 fs pulse widths, a continuously variable broadband range from 680 nm to 1300 nm, and a fixed wavelength of 1040 nm. A broad selection of dedicated models is available to make the most of multiphoton performance, including the MaiTai HP/eHP DeepSee-OL (Spectra-Physics). InSight DeepSee Manufacturer Spectra-Physics MaiTai HP/eHP DeepSee-OL Wavelength covered Manufacturer COHERENT Chameleon Vision I/II-OL Wavelength...

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