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Absorbance Plate Reader Module

Absorbance Plate Reader Module

Absorbance Plate Reader Module

Product catalog summary
Overview: The Opentrons Flex Absorbance Plate Reader Module is an automated solution for absorbance-based assays, detecting absorbance in the 450–1000 nm range. It is suitable for applications such as protein quantification, sample normalization, cell viability assays, and bacterial growth monitoring.
Key Features:
  • Wide Application Range: Ideal for proteomics, cell biology, and microbiology, covering absorbance measurements from 450–1000 nm.
  • Full Walkaway Automation: Paired with the Opentrons Flex Gripper, it automates processes from plate loading to data collection.
  • High-Throughput Capability: Automates multiple plates in a single run, enhancing throughput while maintaining accuracy and reproducibility.
  • User-Friendly Software: Controlled via the Opentrons App, offering an intuitive interface for assay setup, protocol execution, and real-time data analysis.
Specifications:
  • Dimensions: Reader: 9.6 x 15.5 x 5.7 cm; Adapter: 8.6 x 12.8 x 1.7 cm.
  • Weight: Approximately 790 g.
  • Composition: Aluminum.
  • Microplate Type: 96-well microplates (flat-bottomed).
  • Wavelength Filter Settings: 450 nm, 562 nm, 600 nm, 650 nm; custom wavelengths available.
  • Measurement Method: Absorbance with endpoint and kinetic techniques.
  • Detection: 96 photodiodes.
  • Measurement Range: 0–4.0 optical density (OD).
  • Resolution: 0.001 OD.
  • Accuracy and Reproducibility: Detailed specifications provided for different OD ranges and wavelengths.
Application Case Study: A study on protein quantification and normalization using the module demonstrated its capability in handling 40 artificial BSA samples through a fully automated workflow. Absorbance measurements were taken, and protein concentrations were interpolated from a standard curve, with normalizations confirmed by a second measurement.
Contact Information: For sales, email [email protected]; for services and installation, email [email protected]; or call +1 (646) 212-3703.
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Catalog excerpts

Absorbance Plate Reader Module-1

Opentrons Flex® Absorbance Plate Reader Module The Opentrons Flex Absorbance Plate Reader Module is a fully automated solution for absorbance-based assays. Capable of detecting absorbance in the 450–1000 nm range, it is optimized for a broad array of applications, including protein quantification, sample normalization, cell viability assays, and bacterial growth monitoring.

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Absorbance Plate Reader Module-2

Engineered for seamless integration with the Opentrons Flex liquid handler, this module facilitates complete walkaway automation, enabling high-throughput data collection with minimal manual intervention. Paired with the Opentrons Flex Gripper, it automates every step from plate transfer to measurement, ensuring consistent and reproducible results. Protein quantification (BCA/Bradford) ELISA Mass spec Cell viability sample prep and growth assays Wide application range: Ideal for protein quantification, normalization, cell viability assays, and bacterial growth monitoring, covering absorbance...

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Absorbance Plate Reader Module-3

Application case study: Protein quantification and normalization with the Opentrons Flex Absorbance Plate Reader Forty artificial bovine serum albumin (BSA) samples at varying concentrations were created via serial dilution. Protein concentrations from these samples were quantified and normalized in a fully automated workflow on the Opentrons Flex (Fig. 1). Absorbance measurements from the samples and standards were taken (Fig. 2) and protein concentrations were interpolated from a standard curve (Fig. 3). Normalizations were carried out and confirmed by a second measurement using the Plate Reader...

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Absorbance Plate Reader Module-4

Measurement Specifications Detection Unit Specifications Specification Module dimensions Module weight Microplate type Wavelength filter settings 450 nm, 562 nm, 600 nm, 650nm; custom wavelengths available NEED MORE INFORMATION? Trademarks: Opentrons’, Opentrons drop logo, Opentrons Flex* (Opentrons Labworks, Inc.). Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law. OCT 2024 © OPENTRONS 2024. ALL RIGHTS RESERV

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.