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Flex fits your NGS Workflow

Flex fits your NGS Workflow

Flex fits your NGS Workflow

Product catalog summary
Overview: The document introduces the Opentrons Flex NGS Workstation, designed to automate Next-Generation Sequencing (NGS) library preparation. It highlights the collaboration with leading NGS reagent companies to provide verified scripts for library preparation.
Key Features:
  • Automation of NGS sample preparation to simplify workflows.
  • Access to the Opentrons Protocol Library for verified protocols.
  • Custom protocol development available from field scientists.
Installation and Setup:
  1. Download an existing protocol from the Opentrons Protocol Library or request custom protocol development.
  2. Adjust variables such as sample number using the touchscreen interface before running the protocol.
Supported Protocols and Kits: The document lists various manufacturers and their kits compatible with the Opentrons Flex NGS Workstation, including:
  • Beckman Coulter's AMPure® XP for clean-up processes.
  • Integrated DNA Technologies' xGen™ kits for DNA library preparation and normalization.
  • Illumina's range of DNA and RNA prep kits.
  • New England Biolabs' NEBNext® for methylation sequencing.
  • Oxford Nanopore and Pacific Biosciences for long-read DNA prep.
  • QIAGEN's QIAseq® kits for DNA and RNA library prep.
  • Roche Sequencing's KAPA HyperPlus® for DNA library prep.
  • seqWell's ExpressPlex™ for multiplexed plasmid/amplicon prep.
  • Twist Bioscience's kits for DNA library prep and methylation sequencing.
  • Watchmaker Genomics' DNA Library Prep Kit.
Trademarks: The document notes various trademarks associated with the products and companies mentioned.
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Catalog excerpts

Flex fits your NGS Workflow-1

Flex Fits Your NGS Workflow Take the work out of NGS sample prep with accessible automation Choose the Opentrons Flex NGS Workstation for everything you need to automate library preparation TM Get the Flex NGS Workstation installed in your lab, and learn how to set up and calibrate Download an existing protocol for your kit from the Opentrons Protocol Library or get free custom protocol development from our field scientists Adjust variables, such as sample number, before each run using the touchscreen. Then just press RUN We work with leading NGS reagent companies to develop verified scripts for NGS library preparation. Go to the Opentrons Protocol Library for Opentrons and Manufacturer Verified protocols, as well as protocols in use by members of our community. Visit opentrons.com/applications/ngs/ to find out more.

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Flex fits your NGS Workflow-2

NGS Protocols on Opentrons Flex Method Type Automation Script Beckman Coulter New England Biolabs Oxford Nanopore Pacific Biosciences Parse Biosciences QIAGEN Roche Sequencing xGen™ DNA EZ Library Prep Kit xGen DNA MC Library Prep Kit xGEN Normalase™ Module Illumina DNA Prep with Enrichment Illumina DNA PCR-Free Prep Illumina RNA Prep with Enrichment (L) Tagmentation Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus TruSeq RNA Library Prep NEBNext® Enzymatic Methyl-seq Kit QIAseq Pro Targeted DNA Panels QIAseq miRNA Library Kit QIAseq Library Normalizer Kit KAPA HyperPlus® Kit with...

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