High Troughput NGS

Product catalog summary

Overview
The document discusses the Opentrons Flex High-Throughput NGS Workstation, which automates library preparation for up to 96 samples, allowing for full walkaway processing. This system is designed to enhance laboratory efficiency by automating repetitive tasks such as pipetting.

Key Features

Flex Stacker: A modular tip and plate storage system that increases throughput without requiring additional deck space.
Automated Thermocycling and Plate Sealing: Supports multiple rounds of on-deck PCR with the Opentrons Thermocycler and auto-sealing lids.
Flex 96-Channel Pipette: Capable of pipetting complete plates, as well as single- and partial-tip pickup.

Applications

Targeted sequencing
Whole genome sequencing
Single cell sequencing
Bead clean-ups
Pooling and normalization

Performance and Findings
The workstation successfully automated DNA library prep chemistries using mechanical shearing and enzymatic fragmentation at 96-sample throughput. It supports walkaway automation of popular DNA library preparation kits, resulting in high-yielding, consistent libraries.

Deck Layout
The document provides a detailed deck layout for automating library preparation using IDT xGEN MC1 and Twist EF 2.02 kits, highlighting the placement of modules and plates.

Sequencing Results
Table 1 presents sequencing results, including GC content, percent passing filter, quality scores, and percent mapped for samples prepared with IDT xGen MC and Twist EF 2.0 kits.

Product Components
The workstation includes various components such as the Opentrons Flex Robot, Flex Gripper, 96-Channel Pipette, Magnetic Block, Temperature Module, Thermocycler, and more. Additional components can be customized to fit specific workflows.

Support and Services

12-Month Factory Warranty
Opentrons Care and Care Plus service contracts
Installation and setup services
Custom protocol development

Contact Information
For more information, sales inquiries, or support, contact Opentrons via email or phone.

Catalog excerpts

Seamless end-to-end automation of library preparation, enabling full walkaway processing for up to 96 samples

Open the catalog to page 1

Unlock the full potential of high-throughput NGS automation On long lab days, wouldn’t it be nice to have a second pair of hands? What if you could delegate repetitive pipetting tasks to a robot and focus on other things? With Flex High-Throughput NGS Workstation, you can easily automate workflows like these: Targeted sequencing Whole genome sequencing Single cell sequencing Bead clean-ups Flex Stacker Modular tip and plate storage and delivery system that allows you to 5x your throughput without 5x’ing your deck space. Flex 96-Channel Pipette Flex 96-Channel Pipette supports pipetting complete...

Open the catalog to page 2

To demonstrate the effectiveness of the Opentrons Flex High-Throughput NGS Workstation, we successfully automated two popular DNA library prep chemistries, that use mechanical shearing and enzymatic fragmentation respectively, at 96-sample throughput. The Opentrons Flex High-Throughput NGS Workstation supports walkaway automation of popular DNA library preparation kits, resulting in high-yielding, consistent librairies. Yield AVE: 5.6 ng/ul 8.6 ng/ul Peak Fragment Size AVE: 285 bp 303 bp Figure 1. Deck layout for automating library preparation for 96 samples using IDT xGEN MC1 and Twist EF 2.02...

Open the catalog to page 3

Opentrons Flex Robot 1 Opentrons Flex Gripper GEN1 1 Opentrons Flex 96-Channel Pipette (5-1000 pl) 1 Opentrons Flex Magnetic Block GEN1 1 Temperature Module GEN2 with Opentrons Flex Caddy and Calibration Adapter 1 Thermocycler GEN2 with Opentrons Flex Caddy and Calibration Adapter 1 Opentrons Flex Waste Chute GEN2 1 Opentrons Flex 96-Channel Tip Rack Adapter (2 count) 2 Opentrons Flex Deck Expansion Set (4 count) 1 Opentrons Flex Stacker 2 Opentrons Flex Filter Tips, 20 Racks, 50 pL (1,920 tips) 1 Opentrons Flex Filter Tips, 20 Racks, 200 pL (1,920 tips) 1 Opentrons Flex Filter Tips, 20 Refills,...

Open the catalog to page 4

High Troughput NGS

High Troughput NGS

Catalog excerpts

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