Meet the OT-2: Your Personal Pipetting Robot

All scientists, regardless of resources or role, should be able to spend their time designing experiments and testing hypotheses. But a lot of scientists spend too much time doing tedious, time-consuming work, like manual pipetting. We built the OT-2 to change that. The OT-2 is a benchtop liquid handler designed to be affordable, and flexible enough to automate many common applications. With performance equivalent to robots 10x the cost, any life science lab can use the OT-2 to automate their lab work. The OT-2 automates a range of techniques across genomics, protein sciences and other applications,...

The OT-2 is Trusted by Thousands of Scientists Used by 90% of the top 50 research universities and top 10 pharmaceutical companies worldwide “For a robot that does what the Opentrons does, I was quoted $200,000 - 350,000 dollars.” Kaja Wasik, PhD CSO at Gencove “The sky’s the limit in terms of customization you could do... that would be impossible to do on any other commercially available system.” Scott Ficcarro, Research Scientist Farber Cancer Institute “What used to take a technician an hour can now just be downloading an automatically generated protocol.” Nick Emery, PhD Boston University...

GETTING STARTED Getting Started is Easy Thinking Through your Needs If you check one or more of these boxes, consider a demo of the OT-2 Your lab does 5+ hours of wet lab work per week You want to reduce manual pipetting You want accurate and reproducible results You want to improve data quality You want to reduce assay reruns Everything You Need to Automate your Lab, Including Help at Every Step DEMO THE OT-2 Get a virtual demo and discover how the OT-2 can work for your application ORDER THE OT-2 Bundle your OT-2 with the pipettes, hardware and labware you need RECEIVE AND UNBOX YOUR OT-2 Easy...

High-Performance Robotics OT-2 Features HIGH PERFORMANCE Compare to robots 10x the price FLEXIBLE Modular hardware and swappable pipettes OPEN Use your current reagents and labware NUMBER OF DECK SLOTS POWERFUL Beginner-friendly Protocol Designer GUI to advanced Python API SUPPORTED From onboarding to protocol development and troubleshooting, we’re here for you Fast-fill a 96-well plate in 22 seconds2 ACCURACY 15% accuracy at 1 μL 5% precision at 1 μL The OT-2 is an open-source, precision liquid handling PIPETTE CONFIGURATION system that automates various genomics, proteomics, and drug discovery...

Bundled Solutions for our Most Popular Applications Opentrons workstations come with the OT-2 robot, modules, pipettes, and the labware you need to support your chosen application. They also come with included custom protocol development services to support your specific assay needs. The workstations are reagent and kit agnostic to minimize changes to your current workflow. Choose from these workstations, covering some of our most popular applications: OT-2 NGS Workstation For end-to-end NGS library prep up to 24 samples OT-2 PCR Workstation For PCR setup and thermocycling up to 96 samples OT-2...

HARDWARE MODULES Flexible On-Deck Hardware THERMOCYCLER MODULE Opentrons Thermocycler Module is a fully automated on-deck thermocycler with programmable lid and block temperatures. It has performance comparable to machines 5x its price and enables automation of upstream and downstream workflow steps. TEMPERATURE MODULE The Opentrons Temperature Module is an easy-to-use hot and cold plate module that can maintain constant temperatures. Temperature capacity ranges from 4°C to 95°C and can be used with or without the Opentrons thermal blocks. Each temperature module comes with an aluminum block...

Swappable Single- and 8-Channel Pipettes Opentrons pipettes are designed and optimized for use with the OT-2. They provide accurate and reliable liquid transfers from 1 pL to 1,000 pL. We offer both single and 8-channel pipettes with a variety of volume ranges. Single-Channel AVAILABLEMODELS VOLUME ACCURACY PRECISION 1 8-Channel AVAILABLEMODELS VOLUME ACCURACY PRECISION 1

The OT-2 is compatible with ANSI/SLAS compliant labware and automation friendly consumables. Our software has labware definitions for the following brands in our labware library: Our team can guide you through our Labware Creator or create a custom labware definition for you. Contact us to learn more: [email protected] We offer an array of microplates, reservoirs, tubes, tube vials and racks. Check out our catalog at the end of this brochure.

Choose the Method That’s Right for You The OT-2 is adaptable for various biology laboratory workflows. The software that runs the robot through each procedure, known as the protocol, can be created in the four following ways. Protocol Library The Protocol Library provides users with open-source and well-documented protocols. Users can browse and download ready-to-use OT-2 protocols – without needing to write code. The Protocol Library contains hundreds of protocols developed by the Opentrons team. We recognize that workflows vary, and further protocol optimization may be required. So, we provide...

The Protocol Designer does have limitations in user-defined variables, conditional logic, looping steps, and integrating higher liquid handling actions. The Protocol Designer also does not integrate with the Python program. For more complete control, we provide direct software coding with our Python API and a custom protocol development service. Users with Python experience can easily and quickly automate protocols using the Opentrons Python API. The API is a simple Python framework designed to code automated biology laboratory protocols in a way that reads like a lab notebook. The Opentrons...

Reliable Service from a Team You Trust Risk Free Return Policy Opentrons Care If Opentrons hardware does not work for your assay Our premium maintenance plan reduces instrument needs, we offer a risk-free return on eligible hardware downtime by proactively identifying problems before they purchases. Opentrons provides a full refund in the amount occur and providing priority response. This is an annual found on your purchase order, credit card, or pro-forma service package that provides guaranteed SLA time, video invoice, including all associated shipping costs, within a 60- calls for first response...