TRIO.BAS™ AIRBIO ONE RAPID-VIRUS
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ORUM INTERNATIONAL © ALL RIGHTS RESERVED AIRBIO ONE RAPID-VIRUS is an innovative instrument for airborne viable particle sampling. It is specifically designed for total pathogen monitoring of virus, bacteria, molds and yeast. The instrument has 2 different applications: 1) for collecting virus in a liquid sample for subsequent rapid analytical identification (by PCR) 2) for traditional impact on agar culture media plate to count colonies (CFU) ORUM INTERNATIONAL © ALL RIGHTS RESERVED

Description The collection of micro-organisms and virus on culture media requires time for incubation before results. Instead, much faster results are obtained applying the liquid collection method. This method has great advantages when applied in the following fields: • Industrial segment (pharma, agro-food, beverage, etc.) for a quick reaction in identifying the contamination • Military branches for notifying possible biological attacks • Hospitals for finding the correct pharmacological product and treatment for patients • Public institutes (school, restaurant, bar, metro, train,...

1. Remove the s/s aspirating head and cover head from AIRBIO ONE 2. Position the collection liquid system into the AIRBIO ONE RAPID-VIRUS 3. Take out the conical tube from the collection liquid system 4. Fill in the liquid in the tube 6. On the air sampler, select VIRUS under SPECIAL SAMPLING menu and press START 7. When the sample is finished, transfer the conical tube to the laboratory for analysis 8. The sample is analysed via PCR system ORUM INTERNATIONAL © ALL RIGHTS RESERVED

Virus Sampling Protocol AIR SAMPLING STRATEGY: place, time, frequency according to validated SOP AIR SAMPLER PREPARATION: volume of air and type of liquid according to the analytical laboratory AIR SAMPLER PROGRAMMING: air sampler protocol according to user manual and validated SOP SAMPLES COLLECTION: after using a sterile conical tube, place a new sterile tube on the collection liquid system for a new test SAMPLES: PCR test, qPCR RT, qPCR AIR SAMPLER DECONTAMINATION: use 70% isopropyl alcohol NEW SAMPLE: the air sampler is ready for a new test ORUM INTERNATIONAL © ALL RIGHTS RESERVED

Virus sampling procedures Sampling air volumes The suggested volume of air is 2.000 liters. The air sampler has an autonomy of 70.000 litres, but it could be more whist connected to the main power. Sampling place The air sampler should be positioned on the direction of the air flow (between door and windows, close to the air conditioning system of HVAC). In hospital, the sampler should be placed near to patient’s bed, in public spaces where there is the highest concentration of people. Sampling Protocol A specific SOP (Standard Operative Procedure) needs to be prepared. Collection liquid...

ORUM INTERNATIONAL © ALL RIGHTS RESERVED

ORUM INTERNATIONAL © ALL RIGHTS RESERVED

ORUM INTERNATIONAL © ALL RIGHTS RESERVED