Automate your cell- & gene-based therapeutics development and vaccine production VISIT OVIZIO.COM

Benefits The iLine F microscope provides unique online, non- The fluidics system is activated via a unique membrane invasive, dye-free suspension cell counting via accurate, pump, which works in the same way as a heart and has been continuous real-time monitoring. Our algorithms auto- proven to preserve cell integrity. Sterility is assured, and matically detect different cell quality attributes such as continuous cell counting and monitoring can be carried out viability (dead or alive), counting, activation state, and without sample consumption, thanks to the closed-loop The microscope captures...

Accelerate process development & characterization Automation & AI Increase measurement accuracy Increase yield Real-time attributes Improve process & product understanding Reduce costs No waste of biological material Improve waste management

BioConnect & OsOne software The BioConnect can connect to almost any type of bioreactor, including (but not limited to) rocking motion bags, benchtop, single-use/disposable, and even larger scale stainless-steel bioreactors. The OsOne software is at the center experience, OsOne is built The OsOne software uses machine learning to display the different data captured in holograms for easy data acquisition and thorough quantitative data analysis of suspension cell cultures. Cell Quality Attributes (CQAs) • Cell viability The intuitive interface and wizard • Cell count enable a rapid understanding...

Applications Assess cell viability - cell counting T Cell Quality Attributes, directly in the cell culture bag Percentage of Sf9 cells infected by baculovirus in real time Healthy cells are visible as a clear cone of light on a 3D holographic image VIRUS END: Infected cells have multiple lower peaks at the end of the culturing process Track change of status

Specifications Ovizio reader iLine F holographic transmission microscope Cell concentration range [0.1, 25]*106 cells/ml. Note: cell concentration range may vary depending on cell line Light source Partially coherent monochromatic LED 630 nm (red) Average: 3.0 mL/min Maximum 11.6 mL/min Cell travel time Microscope objective Olympus LUCPLFLN20X microscope objective 20x – 0.45 NA Tubing length Total Magnification Tubing diameter Typical tubing size is O.D. 3 mm, I.D. 1 mm Horizontal resolution Internal tubing volume Pump body volume Acquisition rate Retention volume 4.8 mL to 8.3 mL Note: Retention...