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Mosaic
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Catalog excerpts

Mosaic - 1

Next Generation, Digital Illumination ANDOR an Oxford Instruments company Features • Simultaneous illumination of multiple regions of interest • On-board pattern storage for high-speed ROI sequencing • Region specific grey-scaling available for light dosing with 4, 6 and 8 bit per pixel modulation depth • Comprehensive DMD triggering modes • Wide range of light sources - LEDs, lasers and arc lamps • Long lifetime and low maintenance • Supported by SDK and 3rd party software packages with flexible API support and OEM capabilities Benefits • Performs simultaneous imaging and photo-stimulation • Delivers flexible, multi-ROI-specific control of illumination intensity • Augments wide range of techniques: optogenetics, bleaching/activation and uncaging • Ideal for high speed physiological studies • Integrate with electro/optophysiology workstations Photostimulation with Mosaic3For Upright and Inverted microscopes Real Time Parallel Illumination of Multiple Regions Andor’s Mosaic3 is a patented instrument for targeted illumination for microscopy built around MEMS Digital Mirror Devices (DMD). High speed frame switching up to 5,000 fps makes the Mosaic3 suitable for many dynamic applications including bleaching, uncaging, photo-switching, optogenetics and constrained illumination. Mosaic3 exploits DMD in a proprietary programmable platform, integrated with scientific light sources including lasers, LEDs and arc lamps, and operates from 360 to 800 nm. Mosaic3 is available in two models with two different fields of illumination and integrates with both upright and inverted microscopes: 1) Large field models Provide illumination to fill most of the field of view of the microscope and match sCMOS camera FOVs. These models are commonly used for applications such as optogenetics and photoactivation where lower power densities can be delivered by LEDs. 2) Small field models Matches the EMCCD iXon series cameras and are suited to applications including bleaching and uncaging where higher power densities delivered by lasers are required. •6 Duet option - Dual DMD-illumination ports for simultaneous two-color, programmable photostimulation/imaging. For example, regions of interest can be illuminated with an excitatory wavelength and the remaining field with a second inhibitory or imaging wavelength. The on-board memory allows for sequences of patterns to be defined and executed with real-time accuracy under software control or following external trigger signals for ultimate experimental flexibility. A unique feature of the Mosaic family is the greyscale functionality for region-specific illumination intensity control in addition the binary operation of the DMD mirrors. Key Specifications^ 360 nm to 800 nm Minimum Exposure Time 50 ps - 200 ps (Trigger mode dependent)Maximum frame rate 5000 fps (Trigger mode dependent)

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Next Generation, Digital Illumination Cell signalling, Neuronal signalling, Electrophysiology, opsin controlled secondary messengers Cell dynamics, intracellular organelle tracking, cytoskeleton studies Cell signalling, ion imaging, neurotransmission Protein dynamics, protein turnover, cell compartmentalisation Release of Caged Compounds. Mosaic3 can be used with UV sources including arc lamps, LED and lasers to uncage biomolecules and render them active (Eder and Bading, BMC Neurosci. 2007; 8: 57). Photoactivation of pa:GFP-histone in U2OS nuclei lights up histones green in a linear...

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Next Generation, Digital Illumination There are 2 models of Mosaic3 for upright and inverted microscope types*: The Mosaic3 inserts between the epi-illuminator and trinocular head of upright microscope models. Mosaic3 uses the microscope’s epi-fluoresecent port of inverted microscope models. Standard option • Provides illumination within the target region(s). Duet option • The Duet features dual-illumination ports which enables illumination of both the target region(s) and the complimentary area outside the target region(s). • Available on all models apart from large FOI variant for...

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Next Generation, Digital Illumination ANDOR an Oxford Instruments company 6 Technical Specifications" Optical Specifications Transmission Range 360 nm to 800 nm (minimum 5% at all wavelengths)

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Next Generation, Digital Illumination ANDOR an Oxford Instruments company 7 Choosing your Mosaic3 System An overview of the building blocks required to configure a Mosaic3 system is shown below: Please discuss your exact application requirements with your customer representative. Confirm your microscope type rat The Mosaic3 suits many microscopes makes and models. Please specify the microscope details in case there are any requirements specific to your microscope model. Microscope Type Select your required field of illumination Description Large: For large format sensors e.g. iXon Ultra...

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Next Generation, Digital Illumination ANDOR an Oxford Instruments company Order Today Need more information? At Andor we are committed to finding the correct solution for you. With a dedicated team of technical advisors, we are able to offer you one-to-one guidance and technical support on all Andor products. For a full listing of our regional sales offices, please see: Our regional headquarters are: Europe Belfast, Northern Ireland Phone +44 (28) 9023 7126 Fax +44 (28) 9031 0792 Product Compliance Information Regulatory Compliance • EU EMC/ LV/ RoHS2 Directives • Safety-tested under IEC CB...

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