Electroporation Electrofusion & Products
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Electroporation Electrofusion Products Plant & Insect Vaccine Delivery In Vivo, In Utero & In Ovo Mammalian Cell Transfections Mammalian, Oocyte & Plant Large Volume Transfection CRISPR Gene Editing Bacteria & Yeast

Your Complete Electroporation Electrofusion Source has been at the forefront of electroporation technology since we introduced the first commercially available electroporator in 1983. For over 30 years, we have made it our priority to focus only on electroporation and electrofusion. This focus has allowed us to develop the experience and expertise to supply you with a broad selection of innovative in vivo and in vitro tools to advance your research. Our range of electroporation systems and accessories cover virtually every application, including high throughput. Additionally BTX provides...

Comprehensive Online Protocol Database for Electroporation and Electrofusion Research visit: btxonline.com/technical-resources/protocol-database.html Search by system, cell type, transfectant, keyword, or citation COL RATION PROTO T ELECTROPO & ECM® 830 H ons GEMINI™ X2 pal to amProNeur id _ _ n co sm __ a .5) hil : pla __ d H7 . _ op , p the a t PD DTA ole ctan ___ lY by E ed 0 m mM ida sfe __ 50 ow n _ nd in 7.5 , foll Ca r Tra ___ ml pH in g lls/ e: Cl, ak ce 1 yp s-H le sh n o __ .r 08 ut Tri tio ___ nt ll T x1 ate abo -w ca mM h ge n: f 1 Ce ic e n d in pli __ _ tio ty o (10 wit i th...

Table of Contents Applications Electrodes & Chambers Buffers & Accessories

Applications Explore this section to learn about the many applications for electroporation and electrofusion. BTX generally recommends a system that has an exponential decay waveform for bacteria, plant cells, insect cells, and yeast applications, and a square waveform for mammalian cell work.

Bacteria & Yeast Electroporation is recognized as one of the most efficient methods of transforming human genes into prokaryotic cell lines. Researchers use this technique to express recombinant proteins to study gene function and for the therapeutic treatment of human diseases. Typically, the most commonly transformed cell lines are bacteria and yeast, such as Escherichia coli, Agrobacterium tumerfaciens, Pichia pastoris, and Saccharomyces cerevisiae. Electroporation of gram-negative bacterial strains can achieve transformation success rates in the range of IxlO10 transform ants/pg DNA....

Economical Solution The ECM 399 provides the voltage range needed to achieve the field strengths of 12–25 kV/cm essential for efficient transformation. The fixed internal resistance and capacitance settings which deliver the pre-optimized time constants of 5–6 ms in high voltage (HV) are ideal for the transformation of gram-negative bacteria. This system offers the best low-cost solution for simple transformation needs. Prokaryotes and Eukaryotes Solution Labs working with a variety of bacterial and yeast strains often need to transfect mammalian cells as well. This requires more...

Plant & Insect Electroporation For many years, agriculture and horticulture labs have used electroporation to transform plants in order to generate transgenic crops. Electroporation offers an alternative method for the delivery of genes directly into plant cells, plant tissues and plant protoplasts. Electrofusion allows for the fusion of plant protoplasts for transgenically modified plant applications. Transformation of plants can be successfully accomplished without prior removal of the cell wall, allowing for greater genetic manipulation potential of the plant cells. Whether performing a...

Insects Electroporation has been widely used for successfully transfecting insect cells and tissues. Newton Ruiz, et al. effectively utilized ECM 2001+ square wave electroporation as a non-invasive delivery method for dsRNA-mediated gene silencing in Rhipicephalus Microplus eggs and embryos. The BTX generators can be used with our specialty electrodes for tissue specific transfection in insects or BTX microslides can be utilized for the transformation of large numbers of insect eggs. Powerful Exponential Decay Wave Pulse The diverse combination of settings joined with the power of the...

Mammalian Cell Transfection Electroporation is an efficient non-viral method used to transfect genes and other molecules into mammalian cell lines. This technology is commonly used to study gene targeting, function and to understand protein regulation. Electroporation is a standard method used to transfect mammalian cell lines to express recombinant human proteins which are used for therapeutic purposes. Gene delivery by this method is typically used to create a transient transfection in order to study protein expression or to temporarily knockout or “silence” genes using siRNA. This is...

The ECM 830 or Gemini X2 can be coupled with our specially designed HT plate handler, which can transfect up to 96- or 25-well samples quickly and efficiently. This greatly reduces the time to optimize experiments and process large number of samples. Square Wave Electroporation Generator Gene Silencing The use of technologies such as CRISPR/Cas9, siRNA, and morpholinos to analyze gene function are fundamental for research. The ECM 830 has been used successfully for these applications with inhibition and cell viabilities of up to 90%. Callif, et al. recently utilized a high-throughput method...

In Vivo, In Utero & In Ovo Applications The delivery of genes and drugs directly into living tissues has significant implications in gene therapy applications, cancer treatments, vaccine development and transgenic animal production. Tissues and whole embryos can be transfected with the use of specialty electrodes for the following methods: in vivo, in ovo, in utero and ex vivo tissues. Electroporation-mediated gene and drug delivery has been shown to substantially increase intracellular uptake and expression of DNA, siRNA and miRNA in a variety of tissue such as muscle, skin, liver, retina,...