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PCR Biosystems offer a range of best-in-class kits and reagents for PCR and related technologies. By maintaining the highest levels of integrity, professionalism, innovation, and competitive pricing for our customers, we are leading the development of PCR. Our PCR reagents combine enhanced polymerases with highly developed reaction buffers and proprietary hot start chemistry to maximise yield and sensitivity from the simplest to most challenging of reactions. We continuously invest in research and development to bring innovative, high-performing products to market, covering a range of...

CONTENTS Real-Time PCR qPCRBIO Selection Table PCRBIO HS VeriFi™ Polymerase qPCRBIO SyGreen Mix | SyGreen Blue Mix qPCRBIO Probe Mix | Probe Blue Mix PCRBIO Ultra Polymerase PCRBIO HiFi Polymerase qPCRBIO Probe 1-Step Virus Detect qPCRBIO SyGreen 1-Step Kits cDNA Synthesis qPCRBIO cDNA Synthesis Kit UltraScript Reverse Transcriptase UltraScript 2.0 Reverse Transcriptase and cDNA Synthesis Kits RiboShield™ RNase Inhibitor Endpoint PCR PCRBIO Enzyme Selection Guide PCRBIO Taq DNA Polymerase PCRBIO VeriFi™ Polymerase www.pcrbio.com | simplifying research PCRBIO Rapid Extract PCR Kit Isothermal...

Chromo4™, MiniOpticon™ , Opticon™, Opticon™ 2 qPCRBIO Probe 1-Step Go | 1-Step Virus Detect Separate-ROX qPCRBIO Probe 1-Step Go | 1-Step Virus Detect No-ROX qPCRBIO Probe 1-Step Go | 1-Step Virus Detect Lo-ROX qPCRBIO Probe 1-Step Go | 1-Step Virus Detect Hi-ROX qPCRBIO SyGreen 1-Step Detect | 1-Step Go Lo-ROX qPCRBIO SyGreen 1-Step Detect | 1-Step Go Hi-ROX qPCRBIO Probe Mix | Probe Blue Mix Separate-ROX qPCRBIO Probe Mix No-ROX qPCRBIO Probe Mix | Probe Blue Mix Lo-ROX qPCRBIO Probe Mix | Probe Blue Mix Hi-ROX AriaMX, AriaDX qPCRBIO SyGreen Mix with Fluorescein qPCRBIO SyGreen Mix |...

qPCRBIO SyGreen Mix • Sensitive • Specific • Fast qPCRBIO SyGreen Mix combines a proprietary non-inhibiting intercalating dye with the latest advances in polymerase technology and buffer chemistry to give you fast, highly sensitive and reproducible real-time PCR. Antibody-mediated hot start technology prevents the formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity with minimal or no optimisation required. qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences, and is able to...

qPCRBIO Probe Mix • Sensitive • Specific • Fast qPCRBIO Probe Mix is a universal probe kit designed to give superior sensitivity and specificity in all probe-based real-time PCR assays including TaqMan®, Scorpions® and molecular beacon probes. By combining antibody-mediated hot start technology with the latest advances in buffer chemistry we offer market-leading performance with minimal or no optimisation required. qPCRBIO Probe Mix can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences. The enhanced...

qPCRBIO Probe 1-Step Go • Thermostable reverse transcription • Sensitive • Early Ct The kit includes a thermostable and extremely active reverse transcriptase and advanced RNase inhibitor. Antibody-mediated hot start technology prevents primer dimer formation and nonspecific amplification giving highly specific and ultra-sensitive real-time RT-PCR with unrivalled efficiency in multiplex. • High efficiency in multiplex reactions Fluorescence Intensity This universal probe kit is engineered for use on a wide range of probe technologies and can be used to quantify any RNA template including...

qPCRBIO Probe 1-Step Virus Detect • SARS-CoV-2 validated • Ultra-sensitive • Accurate qPCRBIO Probe 1-Step Virus Detect is designed for ultra-sensitive detection of viral RNA using probe-based 1-step RT-qPCR. The kit has been optimised with a high-concentration 4x mix, enabling greater sample input and increasing sensitivity, even when working with small volume reactions. Developed with a high-concentration 4x mix, qPCRBIO Probe 1-Step Virus Detect offers greater sensitivity, enabling more sample to be added, and for smaller reaction volumes to be used with confidence. The kit gives...

qPCRBIO SyGreen 1-Step Kits Features • Thermostable reverse transcriptase 45 °C to 55 °C qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultrasensitive cDNA synthesis and dye-based real-time PCR in a single tube. The kits can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences. qPCRBIO SyGreen 1-Step Detect is designed for sensitivity and is ideally suited to the detection of extremely low copy number targets. qPCRBIO SyGreen 1-Step Go gives the earliest Ct and is formulated for rapid and accurate results from high template...

High quality cDNA synthesis is essential for downstream real-time PCR analysis and successful expression studies. qPCRBIO cDNA Synthesis Kit is an easy-to-use 2 tube system specifically developed to generate cDNA for use in real-time PCR. The reverse transcriptase, buffer system and optimised blend of random hexamers with anchored oligo(dT) primers provide unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. The kit combines a thermostable and extremely active modified MMLV reverse transcriptase with advanced RNase inhibitor to enhance cDNA...

UltraScript Reverse Transcriptase • Thermostable • Flexible • High yield The enhanced thermostability of UltraScript Reverse Transcriptase enables the reaction temperature to be increased up to 55 °C, providing higher specificity and efficient transcription of RNA regions with a high secondary structure. The enzyme is supplied with a 5x buffer containing Mg, dNTPs, stabilizers and enhancers. As oligos are not included, UltraScript Reverse Transcriptase provides the flexibility for users to define their own priming strategy. The enzyme gives exceptional performance with gene-specific...