PCRmax Eco 48
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A Bibby Scientific Company Eco 48 Real Time PCR System Speed. Confidence. Value. Sensitivity. Performance.

Introducing the PCRmax Eco 48 The PCRmax Eco 48 real time PCR system is a high specification, economically priced real time thermal cycler that accommodates a unique 48-well polypropylene PCR plate utilising the same geometry as standard 384-well plates, but only 1/8 of the size. This enables users to dramatically reduce the qPCR reagent volumes compared to traditional 96-well instruments, saving users precious sample, whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity. A minimum volume of 5µl is validated, resulting...

The Eco 48 Workflow The Eco 48 workflow is based on three simple steps: • Load samples into the 48-well plate using the backlit EcoDock and place the plate into the • Run experiment. • The Eco 48 software provides instrument control, data collection, and data analysis. The most common qPCR parameters are automatically in place. These can be easily changed to meet specific experimental requirements. Intuitive icons lead researchers through setup, run and analysis quickly and easily Sensitive optical system delivers precise detection for a range of fluorophores Convenient 48-well format meets...

Eco 48 Thermal Technology qPCR specificity and efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the entire heat block, ensuring that all samples are processed equally. The unique thermal block design of Eco 48 achieves this with a unique heating and cooling system that provides accurate ±0.1ºC temperature control and quickly cycles from one temperature to the next. Speed. Confidence. Value. Sensitivity. Performance. Hollow pure silver block filled with conductive fluid, gold...

Optical Technology The Eco 48 Real Time PCR system contains an advanced highperformance optical system that delivers precise and sensitive fluorescence detection, facilitating all 4 colour multiplex applications. The system is provided factory-calibrated for use with SYBR®, FAMTM, HEXTM, VICTM, ROXTM, Cy®5 and can also be used with any qPCR fluorophores overlapping with these calibrated dyes. For excitation, two panels of 48 fixed LEDs provide excitation energy of distinct spectra, enabling broad range excitation. Each of the 48 LEDs illuminates a specific well location, eliminating the...

Applications The PCRmax Eco 48 is a high performance instrument for demanding applications. Absolute Quantification standard curve Allelic discrimination Relative gene expression Absolute & Relative Quantitation • Absolute quantification standard curves with efficiency calculation. • Relative quantification using the ΔΔCq method with support for multiple reference gene normalization. • Relative gene expression with efficiency control. • Next Generation Sequencing (NGS) library quantification. • Copy Number Variation: insertions, deletions, inversions. • RNA Characterisation: siRNA and...

Speed. Confidence. Value. Sensitivity. Performance. Library quantification • qPCR is the most precise method for quantifying libraries prior to cluster generation. • Works at concentrations below the detection threshold of conventional spectrophotometric • Validation of array and sequencing quality data. • Academic, Government and Corporate laboratories. • Human research, AgriGenomics, Translational, Forensics and many more markets. • Probe, DNA Binding Dye and HRM chemistries. • Gene expression, Viral detection, Sequencing Library Preparation, Genotyping and Mutation Screening

Rapid cycling The Eco 48 has rapid heating and cooling rates. Average ramp rates of 5.5°C/sec reduce overall PCR run times. The typical run time for a 40 cycle PCR protocol is under 40 minutes. In testing, the quickest optimised programme enabled a 40 cycle qPCR experiment to complete in just 15 minutes. Speed. Confidence. Value. Sensitivity. Performance. High throughput Eco 48 thermal control is superior to traditional 96-well peltier systems where thermal efficiency decreases as distance from the peltier increases. With Eco 48, faster and more precise cycling increases sample throughput...

ECO 48 Software The Eco 48 system comes with two distinct software elements. The first, Eco Control, drives the units set up and experimental runs while Eco Study is the analysis package. The Eco 48 software are both open licence, you can install them on as many PCs as you like, and are MIQE compliant. Software EcoControl software. Data Analysis • User-installable, intuitive, easy to use software that • With the Eco 48 system and software, data collection integrates user control, real-time data collection, and is monitored in real time, allowing researchers to access advanced data analysis....

EcoDock The ergonomic plate loading dock allow for easy loading of the plate and is backlit to allow the user to easily visualise which wells have been loaded. • Base with LED backlight • Plate adapter fits standard centrifuge rotors • Includes plate sealing tool What’s in the box Eco 48 Instrument Dock and Squeegee EcoControl and EcoStudy software USB stick Plates (Pack of 50) Plate seals (Pack of 50) Power and Ethernet Cables PCRmax Eco 48 Real Time PCR System, includes Accessories and Software (EcoStudy and EcoControl) Pack of 50 Eco 48 Real Time PCR Plates Pack of 50 Eco 48 Real Time...

Delta Seek qPCR detection kits PCRmax is proud to present our new range of qPCR detection kits. Every assay is expert designed, involving in-depth literature review followed by extensive bioinformatics analysis. The kits are optimised via a strict biochemical validation on synthetic oligos to ensure optimal PCR performance. Every kit operates with the same standardised protocol and cycling conditions, which allows multiple kits to be used on the same plate in the same PCR run. • Designed and manufactured in the UK. • Wide range of 500 qPCR detection kits. • Designed to work with Eco 48 and...