PEPperCHIP ® Peptide Microarrays & Services Antibody Validation • Identify linear & conformational epitopes with highest amino acid resolution • Determine conserved & variable amino acids by full peptide substitution scans • Analyze cross-reactions and off-targets effects of research, diagnostic & therapeutic antibodies • Generate antibody fingerprint profiles for serum biomarker discovery Cross-Reactivity Analysis • Human Epitome Microarray with 29,128 linear human B-cell epitopes • Epitome-wide antibody profiling • Microarray with antigen(s) as overlapping peptides antibodies, serum & plasma amino acid cyclic constrained peptides with max. peptide-peptide overlap • From single to multiple antigens in a single assay amino acids • Identification of essential conserved • 15 amino acid linear or 7, 10 & 13 • Bioinformatic analysis to identify • Exchange of all amino acid positions of a wild type peptide with the 20 natural • Epitope identification with amino acid resolution • Analysis of mono- & polyclonal common binding motifs Epitope Substitution Scan Linear & Conformational Epitope Mapping & variable amino acid positions • Unambiquous identification of epitope lengths & epitope variants • Assessment of antibody selectivity & cross-reactivity Project Initiation Microarray Production PEPperMAP® Service Scientific Reporting • Describe your scientific aim • Microarray design • On-chip immunoassays • Incl. material & methods • Submit your protein sequence(s) of interest • Peptide printing • Microarray read-out • Final quality control • Data evaluation • All experimental details, raw & formatted data • Provide your antibody or serum samples

PEPperCHIP® Peptide Microarrays High density PEPperCHIP® Peptide Microarrays are fully tailored to your scientific needs from single or multiple antigens as overlapping linear or cyclic constrained peptides to large scale peptide libraries or proteome wide analyses. Get access to the most flexible and cost-effective peptide microarray technology from multiple array copies to tens of thousands of peptides per chip. High quality PEPperCHIP® Peptide Microarrays are designed and generated under ISO 9001-certified conditions by peptide laser-printing in only 4 weeks. Discovery Format Mapping Formats...