Immunological Services | Regulatory T-Cells Analyze Treg-mediated immune responses to validate your therapeutic candidates Regulatory T-cells (Tregs) are essential for controlling the immune response and maintaining self-tolerance. Hence, Tregs are promising cellular targets for cancer and autoimmune diseases treatment. While preventing Treg-mediated immunosuppression can help to reinforce antitumor immune responses, forcing an induced Treg (iTregs) phenotype can temper pathologies in autoimmune diseases or transplant rejection. With our iTreg phenotyping service, we can monitor the success of an ongoing clinical trial. Furthermore, via our iTreg polarization assay and iTreg suppression assay, we can support the development of Treg-targeting drug candidates by validating the modulating effect of your therapeutic candidates on T-cell polarization and function. The iTreg polarization and iTreg suppression assays are established for drug screenings in immune-oncology and autoimmune diseases. Applications Monitor in vitro the amount and character of Tregs during patients' treatment in clinical trials. Test the effectiveness of a drug in enhancing the regulatory phenotype and functional character of iTregs, which could be beneficial for treating autoimmune diseases or transplant rejection. Treg Phenotyping Assay Service The Treg phenotyping assay is based on antibody-staining of Treg-specific transcription factors, surface markers, and quantitative analyses by flow cytometry. It allows the characterization of Tregs among the T-lymphocyte population or peripheral blood mononuclear cell (PBMC) samples. Subsequently, the identified Treg population can be sorted for further functional assays. Revise the efficiency of a drug candidate by preventing Treg differentiation and inhibiting iTreg function. This can support cancer treatment by inhibiting Tregs to boost antitumor immune responses. Lymphocyte separation media Treg phenotyping Magnetic for clinical trials cell sorting PEPperPRINT GmbH • +49 6221 7264489 • pepperprint.com • in pepperprint-gmbh sr@pepperchip f PEPperPRINTgmbh OPEPperPRINT

iTreg Polarization Assay Service The iTreg polarization assay is a rapid and reliable in vitro system to quantitatively validate the capability of therapeutic candidates to modulate differentiation of naive T lymphocytes towards an iTreg phenotype, or to verify the candidate compound-mediated prevention of iTreg polarization. Drug candidate Naive T-cells Polarization cocktail Flow cytometry analysis iTreg iTreg Polarization Assay. Naïve CD4+ T cells isolated from peripheral blood mononuclear cells (PBMC) are activated with a Tregpolarization cytokine cocktail. The drug candidates can be screened...