PEPperCHIP® Autoimmune Epitope Microarray The PEPperCHIP® Autoimmune Epitope Microarray provides a perfect platform for discovering autoantibodies, elucidating serum signatures of different patient groups and comparing the signatures with known epitopes and antigens from the Immune Epitope Database (IEDB). Features: • 4,287 linear B-cell epitopes associated with different autoimmune diseases • includes 286 citrullinated peptides and corresponding arginine variants • sequence information obtained from Immune Epitope Database (http://www.iedb.org/) • epitope length: 3 - 51 aa • peptide length: 3 - 17 aa depending on the epitope length; longer epitopes were translated into overlapping 17 aa peptides • sample requirements: 1 - 10 pl serum • HA, c-myc and polio marker peptides routinely incorporated as on-chip QC reference Epitopes related to autoimmune diseases of: • urogenital tract • blood (e.g. antiphospholipid syndrome) • cardiovascular system • central nervous system (e.g. multiple sclerosis) • endocrine and exocrine system (e.g. diabetes mellitus, Sjogren's syndrome) • eye, nose and throat • gastrointestinal tract (e.g. Crohn's disease) • musculoskeletal system (e.g. rheumatoid arthritis) • peripheral nervous system • skin and connective tissue (e.g. vitiligo) • systemic autoimmune disease (e.g. systemic lupus erythematosus) Analysis: PEPperCHIP® Peptide Microarray analysis is straightforward and based on standard immunoassay protocols. PEPperPRINT also provides PEPperMAP® assay and analysis services Material provided by PEPperPRINT: • PEPperCHIP® Incubation Tray: adapted to the array layout, fully reusable, for assays with low sample requirement • PEPperCHIP® Staining Kit: contains labeled anti-HA antibody for staining of control peptides • PepSlide® Analyzer: software solution for peptide microarray image analysis (free 14-day version available) Material and lab infrastructure requirements: • orbital shaker • access to a microarray scanner: PEPperCHIP® Peptide Microarrays are compatible with most microarray scanners (e.g. GenePix 4100B, Agilent or LI-COR Odyssey Scanners) • buffers: PBS, Tween • blocking reagent: BSA, HSA or Rockland Blocking Buffer • labeled secondary antibody for staining of primary samples (e.g. mouse anti-human antibody)

PEPperCHIP® Standard Peptide Microarrays For autoimmune research: • Cyclic Citrullinated Peptide Microarray ^ covers 577 different cyclic constrained peptides, including 335 different citrullinated peptides and the corresponding arginine controls • Human Epitome Microarray ^ covers all linear human B-cell epitopes of the Immune Epitope Database For infectious disease research: • Bacillus Anthracis Antigen Microarray • BK Polyomavirus Antigen Microarray • Dengue Virus Type 1 Proteome Microarray • Dengue Virus Type 2 Proteome Microarray • Dengue Virus Type 2 Antigen Microarray • Dengue Virus Type...