PEPperCHIP® SARS-CoV-2 Proteome Microarray Profile specific antibody responses and identify epitopes against SARS-CoV-2. • Serologically screen antigens of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). • Contains the whole proteome of SARS-CoV-2 (GenBank ID: MN908947.3) including 49 mutations of the B.1.1.7, 501.V2, B.1.1.28 and P.1 Manaus variants, and all spike protein mutations of the Alpha, Beta, Gamma, Delta, Delta Plus and Omicron variants of concern. • Applications - High-resolution epitope mapping of anti-SARSCoV-2 antibodies (IgG, IgA, and IgM). - Identification of SARS-CoV-2 epitope biomarkers for IVD develoment and vaccine research. PEPperCHIP® Pan-Corona Spike Protein Microarray Seven human coronavirus spike proteins. One single assay. • Analyze antibody reactivity against all seven human coronavirus spike proteins. • Library content: spike proteins of SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-HKU1, HCoV-OC43, HCoV-NL63, and HCoV-229E as well as all spike protein mutations of the SARS-CoV-2 Alpha, Beta, Gamma, Delta, Delta Plus and Omicron variants of concern. • Applications - Cross-reactivity analysis of spike proteinspecific antibodies on the epitope level. Assay purified antibodies or sera. PEPperMAP® Epitope Mapping Services Send us your samples and get a full scientific analysis report. All we need to get started is your target antigen sequence and your sample antibody or sera. From epitope identification and antibody validation, to full-scale epitope biomarker discovery, we offer customized solutions for your research needs. How can we support your research? Get in touch with one of our scientists to discuss your project: PEPperPRINT GmbH • +49 6221 7264489 • pepperprint.com •

Patient sera Control Sera Antibody profiles Overlapping peptide library Hi writtfw PEPperCHIP® SARS-CoV-2 Proteome Microarray Fig. 1. Proposed antibody profiling workflow using the PEPperCHIP® SARS-CoV-2 Proteome Microarray. The entire SARS-Cov-2 viral proteome is translated into overlapping peptides and printed onto glass slides. Patient sera is incubated on the chip and antibodies present in the sample bind to epitopes recognized within individual peptides. The resulting response profile may be compared across different samples to monitor B-cell responses over time, or to determine immunodominant...