Application Note - Hindlimb Ischemia model
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Application Note Hindlimb ischemia (HLI) model Human vessels can remodel as a response to different stimuli. The underlying processes are thought to be angiogenesis (proliferation of capillaries) and arteriogenesis (enlargement of preexisting arterioles). Understanding these could potentially result in better treatment options for patients suffering from clinical conditions characterized by insufficient blood supply (e.g. peripheral arterial disease). For this purpose, many researchers use the hindlimb ischemia model, an animal model in which ischemia is produced by femoral artery ligation. It is important to be able to confirm the induction of ischemia and to follow changes in hindlimb perfusion over time. Perimed has a long experience in providing instruments for this purpose. The PeriCam PSI provides high resolution, high quality perfusion images recorded in real-time, with advanced, user-friendly software for acquisition and analysis. Case: hydrogel treatment in a rat model In a study at the University of California San Diego¹, hindlimb ischemia was induced in Female Sprague Dawley rats by removing a 2 cm segment of the femoral artery and vein. Decellularized extracellular matrix-based hydrogels were tested as a treatment. The hydrogels were injected one week postsurgery and perfusion was monitored regularly using the PeriCam PSI for up to 35 days post-injection. Due to the fast nature of the measurements, perfusion could be monitored dynamically during anesthesia. This enabled determination of a new parameter called perfusion kinetics ratio, which gives additional insight into the health of the vascular network by measuring the ability to transition through a change in cardiac output. Both the hindlimb perfusion and the perfusion kinetics showed significant improvement with the treatment. Blood perfusion in rat legs: (1) control and (2) induced femoral artey ligation. PeriCam PSI NR. Courtesy of University of California, San Diego Live recording of the perfusion due to changes in anesthesia. Healthy limb (blue line) and the ischemic limb (red line). Courtesy of University of California, San Diego YOUR PARTNER IN MICROCIRCULATION

Application Note Case: stem cell therapy in a mouse model Male Balb/C mice were used in a hindlimb ischemia model to study stem cell therapy at the University of Miami². Critical limb ischemia was created by excision of the femoral artery above the saphenous branches along the deep femoral artery and below the external iliac artery. Ischemia was confirmed using the PeriCam PSI, which showed a 67% decrease in flow after the excision. The perfusion was then measured 7, 14 and 21 days post-surgery. Treatment with marrow-isolated adult multilineage inducible (MIAMI) cells incorporated in a...