Automated Nucleic Acid Isolation from 200 μl Clinical Sample Material
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Automated Nucleic Acid Isolation from 200 μl Clinical Sample Material 10 9 Yield (EDTA, µg/sample) The isolation of high-quality nucleic acids from whole blood and plasma sample types suitable for your downstream clinical application presents a key bottleneck. High recovery rates, nucleic acid quantity, purity, and degradation directly impact the ability to generate quality results from your testing. PerkinElmer‘s chemagicTM instrumentation and kits offer reliable solutions for this challenge by providing • • • • • • DNA isolation directly from human whole blood or plasma samples, no need for additional treatment on primary samples prior to extraction Ready to use DNA in high yields and purity Fast processing times – 96 samples in < 60 min. Long DNA fragments – up to 200 kb suitable for NGS High recovery rates Autonomous system regarding downstream assays Fig. 1: The DNA yield (EDTA tubes, 41 specimens) from 200 μl sample volume. White blood cell counts of healthy donors were determined and were in the range 4.3 - 8.8 x 10e6 cells/ml. Fig. 2: Purity of the isolated DNA (Absorbance ratio A260/A280) of 41 Citrate and EDTA specimens Europe: For in vitro diagnostic use. The chemagic 360-D instrument meets the requirements of the In Vitro Diagnostic Medical Device Directive (98/79/EC). The CE IVD-registered chemagic 360-D instrument is for distribution and use in specific European countries only. USA: For in vitro diagnostic use. The chemagic 360-D instrument is FDA listed and if used in combination with chemagic kits indicated for use with the chemagic 360-D instrument, is intended for applications described in the resp

Tab. 1: Descriptive statistics. DNA isolation results preformed with the chemagic DNA CS200 Kit and the chemagic 360-D instrument using whole blood samples from healthy donors. Note: The DNA yield is also effected by the sample dilution caused by the used sample tube (the volume of preservative is different in EDTA and citrate sample tubes). Magnetic Separation The magnetic separation is based on the use of metal rods that are lowered into a process solution (A). To collect beads from the solution, the rods are magnetized. Pellets form at the tips of the rods, and the rods are withdrawn...