S P E C I F I C A T I O N S Plate Readers EnSpire Multimode Plate Reader • Label-free Technology • Alpha Technology • Ultra-sensitive Luminescence • Quad-monochromator – Fluorescence Intensity – Absorbance Description The EnSpire® Multimode Plate Reader is a high performance, compact and configurable instrument designed with filter or quadmonochromator capabilities. EnSpire reads optical biosensor label-free, fluorescence intensity, absorbance, ultra-sensitive luminescence and patented Alpha technologies in microplates up to 384-wells. EnSpire has been designed for laboratories with diverse user needs, and includes an intuitive and 'easy-to-use' touch screen to reduce assay start-up times, and simplify protocols across a wide array of applications. The EnSpire is proven to support many different assays including: •  abel-free technology, based on the L Corning® Epic® System, is suitable for non-invasive cellular receptor and signaling assays, as well as biochemical binding assays, such as protein:protein and protein:small molecule interaction assays. • P   hotometric technology enables nucleic acid/protein quantitation, as well as ELISA absorbance assays. •  luorescence technology delivers the F sensitivity required for quantitation assays. EnSpire Multimode Plate Reader •  xpertise in Alpha technology comE bined with instrument innovation delivers the best performance for AlphaLISA/AlphaScreen® detection. •  Ultra-sensitive luminescence technology enables cell growth and reporter gene assays to be run with higher sensitivity and dynamic range; especially important with low transfection rates. Key Features • Corning® Epic® label-free technology • AlphaLISA/AlphaScreen® detection capability with high power laser excitation • Fluorescence intensity detection capability, top and bottom, with quad-monochromator • Ultra-sensitive luminescence, glow and flash • Choice of filter- or quad-monochromatorbased absorbance detection • Integrated computer with touch screen is "easy-to-use", even with gloves • Integrated data analysis software with data export (Excel® or text files) features • Eight barcode identified filter positions • Integrated barcode reading from all four sides of a microplate • Available with optional dispenser or stacker module • 21 CFR Part 11 support

The optional dispenser module adds dual-channel dispensing capability to EnSpire, enabling real-time dispensing of cells and reagents with a minimum dispense volume of 1 µl. The ability to program 2 dispense steps per well is ideal for dual luciferase assays and offers magnetic stirring and temperature control for the reagent containers (up to 65 °C), via the integrated software. The EnSpire stacker module includes 20- or 50-plate magazines that are compatible with our PlateStak™ Automated Microplate Handler to allow efficient manual transfer of plates to the EnSpire. For even greater efficiency,...

Barcode reader specifications Dispenser specifications Code types CODE39 INTERLEAVED 2/5 CODABAR CODE128 Barcode width 0.25 mm (min) Barcode length 50 mm (max), 6 – 20 characters Barcode height 5 mm (min) Empty space at the ends of barcode label 10 mm (min) Bar-space ratio 1/3 non-fluorescent label material Number of injectors 2 Plate formats 6- to 384-well plates Volume range 1 – 475 μl per stroke, multiple dispense strokes can be programmed Dispense volume 1 µl (min) Dispense increments 0.5 μl steps Accuracy 0.1 % @ 350 μl 1.1 % @ 10 μl < 3 % @ 1 μl Precision 0.05% @ 350 μl 1.0 % @ 10 μl Instrument...

PerkinElmer offers an extensive selection of high quality and validated application-focused microplates in 96-, 384-, and 1536-well formats for use with many assay technologies and instrument platforms to enable superior performance. Special coating, packaging and barcoding services are also available upon request. EnSpire Label-free Microplates** Color Black with clear bottom Well bottom elevation 3.3+/- 0.1mm Maximum well volume (384) 82 µL Dimensions SBS standard Maximum well volume (96) 190 μl Molecular weight detection limit >157 Da (assay dependent) Lid compatibility Low profile lid (included)...