HoloMonitor ® M4 – Non-invasive Live Cell Analysis Non-invasive Live Cell Analysis The HoloMonitor® live cell imaging system enables long-term non-invasive analysis of cell cutures within your standard incubator. Cell biologists worldwide use PHI’s label-free cell imager to automatically obtain live-cell morphology, migration, and proliferation data down to a single-cell level in real-time.

HoloMonitor ® M4 – Non-invasive Live Cell Analysis To understand and cure the diseases that still plague humanity, medical science needs new tools that gain widespread scientific use by being affordable to all scientists. Close to 20 years ago this, together with the excitement of working with something entirely new, inspired me and a group of engineers to start developing a technology that would allow cell biologists to non-invasively study cells while being cultured inside the incubator. Sweden is the home of PHI, cinnamon buns, ABBA and technology innovations, including the three-point seat...

Focus on Your Cells Primary human small airway epithelium cells (SAEC) Non-invasive Quantitative Single cell analysis Fits inside the incubator Induced pluripotent stem cells (iPS) Single cell tracking Wound healing of breast cancer cells (JIMT-1) HoloMonitor ® is a live cell imaging system designed to visualize and quantify adherent cells over time. It is gentle to all cell types and can be used to monitor cell lines as well as delicate cell types, such as induced pluripotent stem cells or primary human cell cultures. As cell imaging with HoloMonitor is label–free and harmless to your cells,...

HoloMonitor ® M4 – Non-invasive Live Cell Analysis User-friendly Applications The HoloMonitor ® software (App Suite) is a powerful tool designed to extract quantitative data about your cells from the holographic images captured by the instrument. The App Suite software is intuitive and guides the user through the simple step–by–step work flow, from experiment setup to result analysis. You can also easily create colorful videos and images for peer-reviewed publications. All data can be exported to Excel for further analysis. Guided End-point Assays Guided Kinetic Assays In-depth Analysis Ensure...

Standard Way vs. HoloMonitor® Way Standard cell culture methods require various instruments, from plate readers to microscopes. Experiments require multiple setups and consumables and most readouts are indirect as label intensities are measured. HoloMonitor®, on the other hand, enables real-time label free and quantitative studies of your cells directly in the incubator. All information is directly obtained by monitoring cells without any phototoxic damage or other disturbances. Incubation start 2 h 6 h 12 h 24 h 48 h 72 h 96 h Time requirement Standard Way A new experiment is required for other...

HoloMonitor ® M4 – Non-invasive Live Cell Analysis Defining Your Cells Without Labels HoloMonitor ® is based on quantitative phase imaging enabling non–invasive visualization and quantification of living cells without compromising cell integrity. Unlike phase contrast microscopy, individual cells can be identified using the HoloMonitor technology taking your research to new discovery horizons. Diode laser Reference beam Sample beam Cell culture Parallel laser light Imprinted laser light Image sensor Holographic microscopy creates quantitative phase images by letting a sample beam and a reference...

Technical Specifications HoloMonitor ® M4 Computer Requirements Light source: External laser unit Operating system: Windows 10, 64–bit Cells: Adherent cell monolayer Processor: Intel Core i7 (8th Gen) Cell culture vessels*: 6-, 24-, 96-well, Petri, IBIDI Other: 2 built–in USB ports * For information about recommended vessels please visit phiab.com/holomonitor/ holomonitor-system/ Autofocusing range: 1.5 mm Image capture rate: 1 image/s Image size: 1024 × 1024 pixels Stage travel range: 100×70×10 (x×y×z) Stage repeatability: 5 μm HoloMonitor® M4 dimensions: 290×200×190 mm (w×d×h) Space required...

Copyright © 2022, Phase Holographic Imaging PHI AB / Revision 0017 HoloMonitor ® M4 – Non-invasive Live Cell Analysis NORTH AMERICA Phase Holographic Imaging Skiffervägen 48 224 78, Lund Sweden [email protected] Phase Holographic Imaging PHI Inc. 256 Franklin St., Suite 1702 Boston, MA 02110 USA [email protected] Phase Holographic Imaging PHI AB Skiffervägen 48, 224 78 Lund, Sweden / +46 46 38 60 80 / [email protected] /