Livecyte Kinetic Cytometer Measure the Motion and Morphology of Every CeLL

Imaging Modalities: QPI Ptychographic Quantitative Phase Imaging Livecyte™ utilises ptychography to capture relative phase shift information, allowing high contrast images to be generated using low level illumination. Individual cells can be identified and characterised according to morphological and behavioural characteristics, providing accurate data for quantitative analysis. Fluorescence-like images without the compromise Phase Contrast Label-free imaging using low level illumination allows individual cells to be continuously monitored for weeks at a time, without altering cell behaviour....

Make Better Use of Fluorescent Labels High specification camera and lenses can capture lower intensity images, allowing greater use of fluorescence with a reduced risk of photo-toxicity. • sCMOS camera. • Full illumination from UV to far-red. Livecyte’s Smart functionality can acquire fluorescence channels at different rates. The system automatically corrects the focus for each channel, at every time point, ensuring every image captured is useable. Set fluorescence image capture rates independently from QPI image capture to minimise photo-induced behaviour. Livecyte software automatically correlates...

Every Cell Tells a Story Automated Segmentation of Every Cell No cell population is truly homogeneous. Cell by cell identification allows the extent of heterogeneity in the cell population to be determined. Distinct sub-populations can then be identified and analysed based on cell characteristics, allowing for more specific, realistic and accurate evaluation of cell behaviour. Overcome the limitations of population level metrics Livecyte automatically tracks and analyses thousands of cells, assessing multiple metrics to create a unique phenotypic fingerprint for each cell. Experimental outputs...

Closer to Real Life Smart Incubation – Full Environmental Control • Set temperature from ambient to 45oC ±0.1oC. • Real time monitoring of humidity, temperature, CO2 and O2 at close proximity to cells. • Culture conditions automatically logged for every time point. • Accurately correlate changes in cell behaviour to cell environment. Let the treatments make the difference Long Term Imaging High Resolution Large Field of View Livecyte allows users to keep more cells in view, for longer, improving the likelihood of capturing rare events. Automated correction of the meniscus allows even cells on...

Livecyte Just Works Livecyte is a True Assay-driven Tool Achieve more efficient imaging by utilising every well in any plate, up to 96 well format. Livecyte’s unique meniscus correction, perfect focus technologies and accurate environmental control minimise plate edge effects. This allows cells in every well to be reliably tracked and monitored throughout the experiment, making best use of your laboratory resources. No calibration. No dedicated consumables. No hidden costs. Go from seeding cells to publishable results with Livecyte’s intuitive workflow. Simple three step process guides the user...

Dashboards Application-specific Dashboards Livecyte software automatically combines multi-panel video with multi-parametric data into a single Dashboard, for publication ready outputs. Get the Full Story of How the Cell Population Behaves Combine multiple metrics and behaviour over time to gain a comprehensive profile. One Experiment, Multiple Outputs 4 Example: Motility Dashboard 1. Multi-panel video 2. Start cell count 3. Start confluency 4. Meandering index 5. Speed distribution 6. Directionality 7. Mean velocity 8. Displacement

Livecyte Bundles Go Beyond Traditional Microscopy Livecyte’s bundle format provides the flexibility to develop your imaging capabilities as requirements and resources dictate. Simply choose the bundle to suit your existing needs and supplement with additional functionality as and when necessary. Go to www.phasefocus.com/livecyte/bundles for details. Phase Focus Limited Electric Works Sheffield Digital Campus Sheffield S1 2BJ UK T: +44 (0)114 286 6377 E: [email protected] W: phasefocus.com 2018 © The trademarks Phasefocus and Livecyte, whether registered or unregistered are the property...