IncuSafe CO2 & Multigas Incubators H2O2 DECONTAMINATION TECHNOLOGY When initiated, the unique high-speed decontamination system uses vaporized H2O2, offering time-saving and documented chamber decontamination with complete safety. DECONTAMINATION CYCLE H2O2 Decontamination How does it work? 1. Hydrogen peroxide (aqueous) is converted to vapour using high frequency ultrasonics. During this process, the fan motor remains active, ensuring H2O2 vapour accesses every point of the chamber and the tubing to and from, and the inside of the CO2 sensor. 2. The H2O2 vapour breaks down into hydroxyl radicals naturally. 3. The hydroxl radicals initiate a chain reaction of electron stealing. STEP 1 Preparation Time: 10 - 15 minutes CONTAMINANT DAMAGE CONTAMINANT CELL 1. Remove all interior components 2. Wipe down the inside of the incubator 3. Reposition interior components to specified locations for in situ decontamination 4. Set up the H2O2 generator (MCO-HP)* *Optional Accessory. H2O2 reagent is required for this process. Decontamination Time: Approx. 135 minutes 1. Press the H2O2 button. The chamber will warm up to 45°C for optimum results 3. H2O2 avapour generation starts 4. Interior fan circulates vapor 5. UV lamp reduces H2O2 to water and oxygen STEP 3 Finish Time: Approx. 10 minutes 2•OH H2O2 separates in two hydroxyl radicals Remaining H2O2 and hydroxyl radicals 4. This unstable internal environment leads to death of contaminants. Remaining hydroxyl radicals and H2O2 are resolved to H2O (aqueous) & O2 (gas). The H2O2 decontamination within IncuSafe Incubators achieves at least a 6 log reduction of major contaminants. The full decontamination process takes less than three hours. High Heat Decontamination •OH + OHH2O2 can obtain an electron from an organic source •OH initiates chain reaction of electron stealing 1. Open chamber door 2. Wipe off remaining liquid with sterile cloth 3. Reposition interior components to normal positions DNA is very susceptible to oxidative damage. Since most bacteria have a single chromosome controlling all their life functions, this kind of effect can be detrimental to their normal function. Prokaryotic organisms often lack repair mechanisms to limit such damage, making them more prone to change. For more information, please visit our website: