PROTEOSAVE™ PROTEOSAVE™: FOR PRECIOUS SAMPLE PRESERVATION When biological samples are incubated or stored in a standard tube or plate, biological materials and its properties can be lost due to adsorption to the plastic surface. This is a serious concern for researchers working with precious proteins, antibodies, and peptides. ProteoSave™ is specially designed for reducing biological sample adsorption to the plastic surface. The surface of ProteoSave™ is coated with an ultra-hydrophilic polymer. This ultra-hydrophilic polymer prevents protein denaturation and adsorption which could be inflicted by surface influence (Fig.1, Fig.2). Also, the coated polymer is bound on the surface covalently, so materials eluting from the surface are reduced to a minimum. ProteoSave™ is shown to be the more suitable tube/plate for your precious biological sample preservation (Fig. 2). PRINCIPLE The surface of the Proteosave™ tubes and wells with high density of hydroxyl groups prevent the non-specific binding of proteins, peptides, and cells to the surface (Fig. 1). The coated polymer is bound on the surface covalently, thus elution of materials from the surface is reduced to a minimum.  Reduction of the adsorption of proteins, peptides and cells.  Elution of materials reduced to a minimum due to the covalently attachment of the ultra-hydrophilic polymer to the surface.  Resistance to organic solvents, detergents, and heat (boiling at 100°C 10min.). Hydrophobic surface • Causes protein adsorption on the surface. • Causes protein denaturation and loss of function. Ultra-hydrophilic surface of ProteoSaveTM • Prevents protein adsorption on the surface. • Keeps protein structure and function. Natural Proteins Denatured Proteins Figure 1. Features of Ultra-hydrophilic surface of ProteoSave™ compared with a hydrophobic surface Hydrophobic surface Tube/Plate surface Ultra-hydrophilic surface Tube/Plate surface Figure 2. The number of peptides detected by nanoLC Ultra 2D with TripleTOF®5600 rom Hep3B digested extracts. (Data Courtesy of Masahiro Kamita, Ph.D , National Cancer Center Research Institute, Chemo

PROTEOSAVE™ Organic solvent resistance APPLICATION  High value sample preservation like Proteins, Antibodies, Vaccines etc.  Protein assay- Enzyme catalysis (example Fig.3).  Preparation of dilution series. Reaction efficiency (%) Methods & Material Reaction Containers: PROTEOSAVE™ 1.5mL microtube, Competitor X, Competitor Y Reaction reagent: SGP11 3mM(0.43mg) (TOKYO CHEMICAL INDUSTRY CO., LTD.), Phosphate buffer(pH7.0) 50mM, Endo-M 10mU/mL(0.5mU) (No. A1651, TOKYO CHEMICAL INDUSTRY CO., LTD.) Total Volume：50 μL, Temperature：30 ºC. Reaction timeslot (hours)：1st, 2nd, 3rd, 4th, 5th, 6th, 24th...