Offline Sorter™ Plexon’s Offline Sorter™ (OFS) is the most recognized and trusted offline spike sorting software in the industry today with 1,700+ publications specifically citing the use of OFS as part of their methods. OFS accepts file types from many data acquisition companies and software programs such as those listed in the technical specifications table—including, Plexon’s advanced PL2™ file type. OFS v4 is a powerful, easy-to-use tool for viewing and classifying action potential waveforms (spikes) previously collected from single electrodes, stereotrodes and tetrodes. Spikes can be displayed as points in either 2D or 3D feature space, where a variety of manual, semi-automated, or fully automated clustering techniques can be applied in order to classify (sort) the spikes. OFS can also perform spike extraction on continuouslyrecorded neural data using a variety of different thresholding methods. OFS allows verification of sorting through a variety of displays, and can calculate cluster separation statistics and sort quality metrics. The sorted spikes can be exported in several formats for subsequent analysis. Powerful Waveform Viewing and Sorting R aster displays of spikes and continuous data Overlapping waveform analysis P rintable Sort Summary View with export to PowerPoint® W aveform features and statistics can be easily exported to MATLAB®, Excel® or text file E xport of sorted data to NeuroExplorer® S ave and recall particular sorting instances for a selected channel (useful for comparisons) Time Segmentation, Adaptive Sorting Methods, and Time-dependent Analysis T ime Segments can be named and individually colored and toggled on/off, and can be saved/restored as NeuroExplorer Intervals A bility to display arbitrary (compatible) combinations of continuous and spike data for a channel together in the Timeline View which replaces the need to associate channels during loading of PLX files or during channel remapping I nterval Selection tool can create Time Segments S upport for multiple spike Sources C lusters vs Time View to show how clusters evolve as a function of time Manual cluster selection in 3D feature space using principal component projections, voltage slices in time, or more than 20 other waveform features such as peak, valley, full-width at half maximum, etc. B ox sorting as a pair of time-level windows W aveform selection in time-voltage space T emplate matching algorithm A bility to load multiple PL2 files simultaneously D ivide each file into time segments and view and sort waveforms from each time segment separately S ort Quality View vs Time Segment Graph View to display how the sort quality changes through the file A daptive template sorting, with the templates changing over time to follow the waveform as it evolves T emplates vs Time View to display how the template adapted through the file T etrode and Stereotrode waveform extraction and sorting using any method U nit cross-correlograms and ISI plots W aveform density plots in 2D or 3D in any feature space W aveform alignment I nterval invalidation and artifact removal For more information email [email protected] or call +1-214-369-4957 www.plexon.com www.plexon.c

Includes L-Ratio and Isolation Distance sort quality metrics Includes Lines and Bands as sort methods Scan modes, including ability to scan using different random initial seed clusters Option to use standard deviation or MAD to calculate fit tolerances or band fit tolerances Enhanced automatic sorting methods with systematic scanning through parameter space and graphs displaying the sort quality metric as a function of the parameters that were varied; select the best sorting according to a selected sort quality metric Automatic sort scan results for each channel can be saved to and loaded from...