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An introduction to clear bottomed assay microplates Porvair Krystal series clear bottom plates are now recognised We supply our Krystal clear plates treated for cell culture as industry-leading tools for Pharma drug discovery and with a low pressure gas plasma that produces an even screening. Our standard clear polystyrene bottom plates surface treatment right across the plate. It is particularly have bases just 0.4mm thick and are moulded from highly suited to cell lines which do not grow easily on plastics. Our polished tools to reduce flow lines, artefacts and scratches, plastic-bottomed plates...

Choosing the correct assay plate Choosing the correct microplate for your application can mean the difference between indifferent and great results. There are three basic methods of obtaining useful optical data from microplate-based samples. The simplest method is absorbance measurement. Where greater sensitivity is required, fluorescence measurements are preferred. Sensitivity can be ten times greater than simple absorbance measurements. The third method involves luminescence, a naturally occurring phenomenon exhibited by certain animal and plant species which can emit light. This is a biological...

LIFE SCIENCES SOLID BOTTOM ASSAY PLATES 96-well clear plates For routine adsorption, absorption, ELISA, mixing and storage applications the standard range of 96-well assay plates offers the perfect mix of affordability and high quality. Manufactured from high quality crystal polystyrene Flat bottom for spectrophotometric work V-bottom minimizing residual liquid Round (U) bottom for cell/particulate collection Robot compatible Working volumes from 275pl down to 10pl Plate description Sterile Colour Qty/pack Cat. no. 96 wells of 350pl with a flat bottom 96 wells of 270pl with a round bottom 96...

LIFE SCIENCES 96-well black, white & black with white wells Porvair Sciences range of solid bottom 96-well assay plates is specifically designed for absorbance, fluorescence, luminescence and scintillation applications. The design uses the most popular 96-well format with standard ‘chimney' wells to overcome optical crosstalk and contamination. These plates are designed to give optimum results from any instrument which reads from the top of the plate. The acclaimed composite black/white plate has been shown to be ten times more sensitive than a standard white plate for chemi-luminesence assays....

LIFE SCIENCES SOLID BOTTOM ASSAY PLATES 384 square-well plates 384-well assay plates from Porvair Sciences are available in black, white and clear. These have been optimised for fluorescence, luminescence/scintillation and ELISA/turbidity measurements respectively. The working volume is 120pl/well. All 384-well assay plates are available in standard and tissue culture treated formats. The 384-well format, with its higher density of wells, allows more work to be carried out in a standard sized plate, aiding higher throughput. All of these plates are manufactured from high quality polystyrene....

LIFE SCIENCES SOLID BOTTOM ASSAY PLATES 24-well Krystal™ black & white Clear bottom plates allow the cells grown on the bottom surface to be viewed using an inverse microscope. In addition, light-emitting assays can be measured from the bottom. Porvair Sciences collection of clear bottom 24-well Krystal plates are optimised for fluorescence and luminescence. The special design of the plate virtually eliminates well-to-well light crosstalk inherent with other clear-bottomed microplate designs, enabling unmatched accuracy, higher sensitivity and better repeatability of photometric readings. For...

LIFE SCIENCES CLEAR BOTTOM ASSAY PLATES 96-well Krystal™ black and white Porvair Sciences clear bottom 96-well Krystal plates are optimised for fluorescence and luminescence. Proprietary ultra-sonic welding is used to assemble the clear bases to the solid top plate in our clean room. This design provides flat, optically clear bases to each well with no leakage, enabling excellent accuracy and reproducibility of photometric readings. All tissue-culture treated plates are supplied lidded and sterile, in individual bags. • Opaque walls to prevent well-to-well crosstalk • Clear plate bottom permits...

LIFE SCIENCES CLEAR BOTTOM ASSAY PLATES 384-well Krystal™ black and white The Krystal 384-well microplate range incorporates novel square wells with a large liquid capacity of 120pl per well, increasing the achievable sensitivity of most HTS assays. Using a proprietary manufacturing technique, the Krystal 384 offers market leading plate flatness (+/- 0.1mm tolerance) that translates into a significant increase in measurement precision and elimination of read errors when performing cell based assays using fluorescent or luminescent imaging. All tissue-culture treated plates are supplied lidded...

LIFE SCIENCES 96-well Krystal™ 2000 black and white The unique Krystal 2000 plate range from Porvair Sciences has been optimised for luminescence and fluorescence assays. A patented manufacturing process, using a ‘two-shot' mould, provides clear, individual wells in an opaque matrix. The special design of the plate totally eliminates the well-to-well optical crosstalk inherent with other clear-bottomed microplate designs, giving unmatched accuracy, sensitivity and repeatability of photometric readings. Very high signal-to-noise ratio and low detection limits can be achieved with this superb plate. All...

LIFE SCIENCES CLEAR BOTTOM ASSAY PLATES Krystal™ glass bottom plates Manufactured for whole-plate CCD imaging and laser detection applications, Krystal glass bottom plates consist of a polystyrene upper part and a clear borosilicate glass sheet fixed to the base with a proprietary adhesive. This process results in consistent flatness of the base and gives improved light transmission whilst maintaining a flat optical plane for growing cells. The nominal cut-off wavelength of 335nm allows most fluorescence assays to be excited or read through the glass bottom. All plates are supplied lidded. 175ym...