Welcome to the first edition of the Porvair Sciences Chromatography & Sample Preparation Catalogue. Whatever your application, we have a microplate solution to help you improve productivity, increase sensitivity and ensure traceability of samples. We are committed to producing top-quality microplates that give reproducible results every time, using only carefully selected plastics and polymers that will not affect your assays. Our plates may cost a little more than the commodities on offer from low-cost producers in emerging nations, but our attention to materials selection, superior moulding...

Biological Sample Preparation for LC or GC/MS analysis Protein Removal using Microlute™ P3 Phospholipid Removal using Microlute™ P3PL Porvair filtration plates Chemical Sample Preparation for LC or GC/MS analysis Supported Liquid Extraction / Diatomaceous Earth Solid Phase Extraction Microplates IRIS polymeric SPE microplates LC/MS Sample Preparation accessories Sample collection plates Sealing caps for microplates Vacuum manifolds Solvent concentrators / evaporators MiniVap® sample concentrator MiniVap® Gemini sample concentrator Ultravap® Levante concentrator Ultravap® Mistral concentrator...

THE COMPLETE 96-WELL SAMPLE PREPARATION SYSTEM FROM PORVAIR SCIENCES For more than twenty years, Porvair's Microlute™ 96-well SPE plates have been helping scientists increase the throughput of their analytical laboratory by speeding up sample preparation. With a range of quality polymeric sorbent materials available, including cation- and anion-exchange resins, Microlute™ sets the industry standard for 96-well plate-based solid phase extraction. The complete Microlute™ system provides a matched filter plate, vacuum manifold and choice of collection plates with the option of a dedicated sample...

B I O L O G I C A L S A M P L E P R E PA R AT I O N F O R L C O R G C / M S A N A LY S I S P R OT E I N R E M OVA L U S I N G T H E P O R VA I R S C I E N C E S M I C R O LU T E P 3 P R OT E I N C R A S H P L AT E Biological samples commonly contain proteins that interfere with downstream applications. The P3 plate uses the ‘crash’ method, in which the protein is denatured with acetronitrile and the flocculant filtered out, allowing 96 samples to be handled at one time. The Protein Precipitation Plate, P3, is based on the industry standard MicroLute™ format, but without the chromatographic sorbent....

New 96-well high throughput plates from Porvair Sciences for ultra-pure DNA purification offer speed and convenience to S EE PAGE 24 FOR O RDERING INFOR MATIO N l DNA samples ranging from 50 bp up to 23 kb can be purified with up to 98% recovery Up to 50 µg of DNA can be recovered efficiently and quickly using a Porvair Sciences DNA Purification Kit your DNA clean-up process. Full DNA extraction kits are also available – please refer to the Chromatrap website www.chromatrap.com For DNA purification, using specialised filtration media offers much higher loadings of active material and reduces...

Supported-liquid-liquid extraction is a separation technique The proprietary frit technology ensures that samples will not using an inert scaffold – usually diatomaceous earth – to drip- or break- through until pressure or vacuum are applied, support one liquid phase whilst a second liquid phase flows allowing longer dwell times on the columns. over it. In this way liquid-liquid separations can be performed S EE PAGE 24 FOR O RDERING INFOR MATIO N without the difficulties of separating the two liquids after extraction. Standard ANSI/SLAS format The new Porvair SLE plates are based on well-understood...

Solid phase extraction (SPE) is a method of sample preparation that concentrates and purifies analytes from solution by sorption, followed by elution of the analyte with a solvent appropriate for instrumental analysis, such as LC-MS. Porvair Sciences offers a complete range of products to implement SPE. MicroLute™ is the original 96-well plate format for SPE. This device consists of a polypropylene 96-well plate loaded with a choice of sorbent and sorbent volumes. The frits have a mean pore size of 20 microns and are made from microporous high-density polyethylene. l Choice of novel polymeric...

Excellent recoveries thanks to the highly retentive nature of polymeric phases An absence of residual silanol groups associated with silica media A high surface area that results in higher analyte capacity compared to silica-based SPE Benefiting from the absence of leachates and extractables, from the plastic and the media Inert to a wide array of solvents and works across a wide pH range Reduced resin volumes for improved detection limits and lower hold-up volumes Six phases to cover most applications IRIS™ sorbents are available in six different phase chemistries offering maximum flexibility...

CHOICE OF SORBENT FOR EACH APPLICATION IRIS WCX (Weak Cation Exchange) IRIS PLUS (100% DVB): Highly retentive reverse phase SPE • 100% divinylbenzene (DVB) phase • Features reduced swelling and increased retention over (PS-DVB) phases • Superhydrophobic for highest retention • Excellent for environmental samples • Available in 25-35 pm or 55-65 pm particle sizes • Organic acid on divinylbenzene support • Pore size: 80A • Ion-Exchange capacity: ~1 meq/g • pKa ~5 • Available in 25-35 jm or 55-65 jm particle sizes • Available in various cartridge sizes and 96-well plates Example of applications:...

The hyphenated techniques of HPLC-MS and all the variations thereof have become accepted methods for the analysis of small biomolecules in many areas - toxicology, pathology, forensics, metabolomics and now, with peptides, genomics. Throughout all these disciplines it is vitally important to maintain clean labware at each stage. The increases in sensitivity that have been accomplished by the very latest Time of Flight Mass Spectroscopy systems have made even the most minor contamination a serious problem for analysts. This contamination may, in fact, not be external to the labware, but contained...