Product Catalog iFlow™ Cell Perfusion System Organ-On-a-Chip (OOC) System

iFlow™ Perfusion Systems Key Benefits High Performance & Reliability Flexible Control Modes Multi Perfusion Modes Cost Effective Simple Setup Droplet cell culture on chip Cell response to medium change Live cell imaging 3D cell culture Stem cell assays Shear stress experiments Drug screening Toxicity tests Calcium imaging Bioreactor research Drug response studies In

Multi-Reagent Perfusion System When used with our rotary selection valves, PreciGenome's PG-MFC pressure controller can allow multiple reagent switching, which is perfect for organ-on-a-chip and cell culture. Our rotary valves have a dead volume of only 4.5 µl. Precise flow rate control can be achieved by an optional liquid flow sensor. System Contents iFlow controller PG-MFC-8CH SwitchEZ rotary valve (6, 8, 10, 12, & 16 available) 2x reservoir kits (1.5, 15, or 50 ml) PG-LFS flow sensor (assorted full scale, optional) Tubings & fittings System Schematic Collection Flow Sensor PG-MFC Touch Reservoir...

Recirculating Perfusion System PreciGenome’s PG-MFC pressure controller can allow a recirculating perfusion system when used with a rotary switching valve, which is perfect for shear force studies on cell culture. Such systems can be configured for unidirectional/bidirectional flow. Precise flow rate control can be achieved by an optional liquid flow sensor. System Contents iFlow controller PG-MFC-8CH SwitchEZ bidirectional 3-way/2-position rotary valve 2x reservoir kits (15, 50, or 100 ml) PG-LFS flow sensor (assorted full scale, optional) Tubings & fittings Address: 2176 Ringwood Ave., San...

Perfusion System Extended Runtime UI Software and API library for complex process control and real-time data monitoring With the iFlow software’s advanced scripting, users can create looping protocols that run for over a week with no drop in performance or accuracy. Nonrecirculating perfusion systems (not pictured) are also capable of these extended runtimes. Address: 2176 Ringwood Ave., San Jose, CA, USA Visit us at www.precigenome.com/nanoparticle-synthesis

Microfluidic Chips Perfusions chambers are a critical aspect of cell perfusion, especially for live-cell imaging. They have to meet two important requirements: maintain cells in a healthy state and allow the living cells to be observed with the highest possible resolution. A wide range of commercial perfusion and imaging chambers are now available. It includes conventional perfusion chambers, i.e. glass bottom Petri dishes, multi-well chambers mounted on microscope slides, heating stages with a variety of interchangeable perfusion adapters, or microfluidic chips. Depending on your experiment,...

Key Factors for Perfusion System Selection Media Recirculating or Non-recirculating System It depends on the whether the cell to cell chemical interaction is essential or not in your experiment. Recirculation perfusion system uses a given volume of culture medium and recirculates the medium throughout the perfusion flow. By using the same culture medium, the molecules secreted by the cells are kept in the same culture flow. Non-recirculating perfusion system can be used for experiments where cell-cell chemical communication is not important or these chemical cues should be ruled out. This perfusion...

iFlow™ Organ-on-a-Chip System

iFlow™ Organ-on-a-Chip Systems Organ-on-a-Chip (OOC) technology provides a novel in vitro platform with a possibility of reproducing physiological functions of in vivo tissue, more accurately than conventional cell-based model systems. The technology opens up great opportunities for next-generation experiments of mimicking human organ functionality, microphysiology and morphology in vitro, replacing traditional animal-based model systems. By realizing different organ functions on a chip, Organ-on-a-Chip technology is potentially useful for building models of complex diseases. The technology can...

Organ-on-a-Chip Chips The chips contain two cavities, each comprising an integrated membrane serving as cell culture area. Cells cultured on the membrane are perfusable independently from both the apical or basal side through upward directed (upper perfusion) and downward directed (lower perfusion) channels. The chips have two in- and outlet ports above and below the membrane. The chip is made by injection molding from polystyrene (PS) or Topas. A 12 μm thick polyethylene terephthalate (PET) membrane (TRAKETCH) with a pore diameter of 8 μm or 0.2 um and a pore density of 1X10e5 pores/cm² was...

Culture Modes and Applications Mode #1: Single-sided Culture A cell monolayer is cultivated on one side of the membrane in the chip. Application Examples of Single-sided Culture Skin-on-a-Chip Model Skin Models with air-liquid interface are a good fit for this culture mode. Skin-on-a-chip (SoC) are used for testing of topical pharmaceuticals and cosmetics, studying the pathology of skin diseases and inflammation. Also it is to test for the presence of antigens or antibodies which could denote the presence of a pathogen. The model in the figure shows a polarized skin cell monolayer is cultured...

Mode #2: Double-sided Culture Two cell monolayers, one layer on the top of membrane and another layer beneath the membrane, are cultivated simultaneously in the chip. Molecules are transferable through the pores on the membrane. Thus, this culture mode is fit for cell studies such as signaling, co-culture, and transport studies. Lung-on-a-Chip Model The lung-on-a-chip is a complex, three-dimensional model of a living, breathing human lung on a microchip. The device is made using human lung and blood vessel cells and it can predict absorption of airborne nanoparticles and mimic the inflammatory...

PreciGenome’s iFlow pressure controllers can be purchased with bundles for organ-on-a -chip applications. The iFlow controller’s combined vacuum/pressure functionality make it perfect for culture media recirculation. Liquid flow lensoi tsftiud Flow sensor Chip cross section Flow sensor null System Contents iFlow controller PG-MFC-8CH 2x reservoir kits (1.5, 15, or 50 ml) OOC membrane chip (optional) PG-LFS flow sensor (assorted full scale, optional) ^SwitchEZ rotary valve (PG-OOC-X-REC only) 2x SwitchEZ 3-way valves (PG-OOC-LT2-REC only) Tubings & fittings Address: 2176 Ringwood Ave., San Jose,...