Product Catalog NanoGenerator® Nanoparticle Synthesis for LNP & PLGA

Nanoparticle synthesis via microfluidic mixing has superior control of size, homogeneity, and repeatability to conventional batch synthesis methods. Two streams, aqueous and solvent, meet in a narrow mixing channel, forming nanoparticles with payload encapsulated. Microfluidic Mixing System PreciGenome’s NanoGenerator® platform uses pressure-based microfluidics for reliable nanoparticle synthesis at several production scales. It has been widely used to produce various types of nanoparticles, such as lipid nanoparticles (LNPs), liposomes, PLGA nanoparticles, etc. Controllable particle size High...

Path from Discovery to Commercialization NanoGenerator® offers controllable and reproducible mixing conditions, ensuring the accurate synthesis of LNPs through its scalable architecture found in the entire NanoGenerator® product line. Options are available for all production stages, allowing seamless transfer of crucial process parameters and guaranteeing consistent critical quality attributes (CQAs). LNPs produced from NanoGenerator® may be used for a wide range of applications, such as vaccine development, gene therapy, cell therapy, etc. Discovery & Screening Flex-M 1 - 12mL per run Flex-M...

NanoGenerator® Flex-S Nanoparticle Synthesis System The NanoGenerator® Flex-S is designed for small scale production. It may run 1 to 4 samples at once, each sample from 0.1 to 0.5 ml. The throughput volume is therefore 0.1 to 2 ml per run, saving over 80% in reagent cost versus larger volumes and perfect for formulation screening and early discovery. Even smaller output volumes (<0.1 ml per run), custom total flow rate, and custom flow rate ratio are attainable at special request by recipe optimization. eGFP mRNA LNP Delivery to Jurkat Cells Example of Formulation Screening by Flex-S Screening...

Flexible Parameters of Flex-S 100 New features of NanoGenerator® Flex-S: • More total flow rate settings. Users can choose 3 or 4 ml/min to conduct LNP synthesis. Higher total flow rate generates smaller LNPs. Other total flow rate and flow rate ratio are attainable at special request. LNP size and PDI also depend on other factors such as the payload and formulation choice. • Output volume as low as 100 ^l is attainable by loading 75 ^l aqueous samples (e.g. mRNA samples) and 25 ^l lipid formulation. Multi-sample Synthesis by NanoGenerator® Flex-S: • 10 seconds, 4 samples! Users can enable multisample...

NanoGenerator® Flex-S Plus Nanoparticle Synthesis System The Flex-S Plus System facilitates the rapid screening of nanoparticle formulations and early-stage mRNA candidates, offering a substantial increase in project efficiency. With a max throughput of 48 samples per run, 96 samples within 90 mins, the Flex-S Plus greatly streamlines screening processes. It offers comprehensive automation of complex protocols, enabling users to concentrate on other laboratory duties. NanoGenerator® Flex-S Plus Library prep functions enable lipid/payload formulation prep from raw stock materials before the LNP...

Automated High-throughput Formulation Screening & Discovery High throughput Screening Workflow Automated Process Workflow Mixing Synthesis Solvent Removal Email: [email protected] | Tel: +1-408-708-4602 | 2176 Ringwood Ave, San Jose CA, USA | www.precigenome.com

Formulation Screening & Discovery with High-throughput Application — 32 sample Screening by NanoGenerator® Flex-S Plus The following shows an application study of 32-sample screening by NanoGenerator® Flex-S Plus with total flow rate of 3ml/min and flow rate ratio of 3:1. Each cell in the heatmap indicates a unique LNP sample with a specific lipid formulation or a specific N:P ratio. The following heatmap offers an insight of the physical properties (size & PDI) of 32 kinds of different LNPs. 32 sample screening — Size (nm) Multi-sample Screening by NanoGenerator® Flex-S Plus Example screening...

NanoGenerator® Flex-M Nanoparticle Synthesis System The NanoGenerator® Flex-M is designed for small to medium scale production. It has a throughput range from 1 to 12 ml, suitable for a variety of applications from early screening to animal studies. An add-on package is available for in-line dilution, allowing users to instantly reduce ethanol concentration during production. This further stabilizes LNPs right before collection. Tunable Flow Rate Ratio 100 Flow Rate Ratio (AqiLipid) Fxied TFR 5ml/min Email: [email protected] | Tel: +1-408-708-4602 | 2176 Ringwood Ave, San Jose CA, USA |...

NanoGenerator® Flex-M Premium NanoGenerator® Flex-M Premium Nanoparticle Synthesis System The NanoGenerator® Flex-M Premium can be upgraded from Flex-M with a hardware installation kit and software upgrade. It has a throughput range from 1 to 200 ml, suitable for a variety of applications from early screening to animal studies and pre-clinical applications. An add-on package is available for in-line dilution, allowing users to instantly reduce ethanol concentration during production. This further stabilizes LNPs right before collection. Email: [email protected] | Tel: +1-408-708-4602 |...

Case Study: T Cell Transfection by mRNA Lipid Nanoparticles Since the first FDA approval of chimeric antigen receptor (CAR) T cell therapy in 2017, T cell engineering has continued to be the hottest research field in immunotherapy and cell therapy. Current CAR T cell engineering methods use viral transductions, which induce permanent CAR expression and have potential safety concerns. To overcome these concerns, researchers are highly interested in non-viral gene delivery methods. Recently, CAR mRNA LNPs in T cell engineering have been widely studied. The transient transduction of mRNA LNPs make...

NanoGenerator® Max Nanoparticle Synthesis System With PreciGenome’s microfluidic technology, customers can seamlessly transfer early discovery results (NanoGenerator® Flex, Pro) to the late stage production (NanoGenerator® Max). The NanoGenerator® Max RUO version can be used for preclinical applications of LNP synthesis, while the NanoGenerator® Max GMP version is designed for clinical and commercial production. High Encapsulation Efficiency Model cGMP compliance Software (21 CFR Part 11 compliant) Throughput Flow rate ratio Inline dilution Size range Encapsulation efficiency DNA, mRNA, siRNA,...