Microfluidic Solutions Provider Stainless screw Bubble TrapIntroduction: Air bubbles in the fluidic flow can cause significant problems for many applications because the bubbles change the intrinsic physical properties of the microfluidic environment unexpectedly. They could increase the internal pressure abruptly in a fluidic/microfluidic system. It may lead to important shear force variations, resulting in changing the compliance of the system or blocking channels in small size. It is recommended to adding line bubble traps in the fluidic system to avoid and minimize the unintended introduction of bubbles into the fluidic flow stream. PreciGenome inline bubble trap is able to remove bubbles in the fluidic flow stream with or without vacuum assistance. It effectively avoids blocking by air bubbles downstream in small size channels. The Trap uses a micro-porous, hydrophobic PTFE membrane held between two PEEK ™ plates. When a fluid stream containing bubbles flows through the unit, the bubbles are forced through the membrane as the pressure within the system is higher than atmospheric pressure outside. The hydrophobic properties of the membrane retain the aqueous fluid within the Trap. Because the membrane function depends on its hydrophobicity, the Trap is only suitable for use with aqueous systems and NOT with organic solvents. Membrane PTFE PEEK® retaining plates 1.For optimal bubble removal the bubble trap should be mounted as shown in the Figure below. Benefits: ♦ Effective removal of air or gas bubbles ♦ Inert materials (PEEK™ body and PTFE membrane) for chemical resistance) ♦ Withstand pressure up to 30psi difference ♦ Fitted to your volume application (internal volume: 25uL, 95uL or 300uL) ♦ Easy membrane replacing to avoid contamination ♦ Compatible with standard 1/4”-28UNF fitting A). this face should be vertical; B). two fluid ports should be positioned at the lowest point which facilitates removed bubbles (gas) move to the top portion of the air path. 2. Connect tubing to the 1/4 -28UNF female threaded fluidic ports. Inlet and outlet are interchangeable. 3. To obtain the maximum bubble/gas removal effect, a vacuum source can be connected to the vacuum port. Address: 2176 Ringwood Ave. San Jose, CA, USA, 95131 Email: [email protected]

Microfluidic Solutions Provider Membrane Replacement: Do not disassemble the product until replacement of the membrane is required. Disassembly and reassembly without replacing the membrane is likely to result in leakage. 1) . Unscrew the retaining stainless screw to separate the tap’s components. 2) . Replace the membrane with a new one. 3) . Place a replacement membrane onto the half of the Trap that contains the fluid ports. If text is present on the membrane, this must face away from the fluid ports 4) . Assemble the two halves of the Trap together with the screw. Tighten the screw hand tight...