Product Catalog iFlow™ Droplet & Single-Cell Microfluidic Systems & Solutions

iFlow™ Droplet System -Touchscreen Version Microfluidic droplet generation allows greater control over size and uniformity than conventional batch production. PreciGenome’s droplet generation package provides a convenient open platform compatible with a wide range of chip designs. It may also be configured for either small volume production (<0.5 ml) or large volume (1.5 ml - 1 L). Single and double emulsion configurations are both available, and the iFlow Touch pressure controller provides a convenient AIO control and display unit. Key Benefits High Performance & Efficiency • Tunable size (chip...

Single Emulsion Pack Oil Phase Water Phase Collection PG-MFC Touch Controller Flow Sensors (optional) High Speed Imaging System (optional) In single emulsion, one dispersed phase forms uniform droplets within the continuous phase. This setup is for water-in-oil emulsion. Oil-in-water emulsion would switch the two phases’ positions. Double Emulsion Pack Oil Phase Water Phase 1 Water Phase 2 Collection Flow Sensors (optional) PG-MFC Touch Controller High Speed Imaging System (optional) In double emulsion, an additional continuous phase encapsulates the initial droplets formed in the first emulsion...

iFlow™ Droplet System - Light Version PreciGenome’s droplet generation package is also available with an iFlow Light pressure controller. This setup is more affordable and compact. External pressure source and display unit are required for full functionality. System Contents PG-MFC-LT-2CH light pressure controller 2x reservoir kits (1.5, 15, 50, 100, or 1000 ml) 2x reservoir tube racks (15 or 50 ml) 2x droplet generation chips (assorted models) PG-LFS flow sensor (assorted full scale, optional) Tubings & fittings Diagram for Single Emulsion PG-HSV high speed imaging system (optional) PG-MP1 mini...

Working Principle The system requires two or more unmixable liquid phases, referred to as the dispersed phase, sometimes called the droplet phase, and the continuous phase. The schematic below illustrates the device with junction in focused-flow geometry designed for droplet generation. Continuous phase Schematic illustration of enlarged junction, controlled break-up droplet at the orifice. The flow through the orifice enables a controlled droplets break-up, which is required for yielding monodisperse micro-emulsions. Dispersed phase Continuous phase Microfluidic Droplet Generator Chips PreciGenome...

Droplet-based PCR DNA/RNA sequencing Drug delivery Protein crystallization Cell culture Chemical synthesis Microparticle synthesis Gel particle synthesis Droplet-based PCR Digital PCR system as a new generations of Polymerase Chain Reaction (PCR) system has been an important tool in genomics and biological fields. Droplet PCR operates by assembling ingredients, forming droplets, combining droplets, thermocycling, and then processing results by using water-in-oil systems. DNA/RNA sequencing Droplet-based microfluidic systems have been used for DNA/RNA sequencing Drug Delivery Droplet microfluidics...

With its user-friendly interface and tunable pneumatic pumps, the iFlow pressure controller allows fine control over pressure ratios to achieve a wide range of droplet sizes and production rates with high consistency between different scales of production. Two-Inlet Chip (15 ml Reservoir Kits) Two-Inlet Chip (500 pl Chip Tanks) Three-Inlet Chip (15 ml Reservoir Kits) Three-Inlet Chip (500 pl Chip Tanks) Address: 2176 Ringwood Ave., San Jose, CA, USA Visit us at www.precigenome.com/microfluidic-droplet-generator 7

Flow™ Droplet System Comparison Chart Product Model Small volume pack with 0.02 to 0.5mL throughput (optional) Large volume pack with >0.5ml throughput (optional) Integrated Software & Computer Integrated Pumps Liquid Flow Sensor (optional) High-speed Imaging (optional) Frame Rate of Imaging Installation, Training, Address: 2176 Ringwood Ave., San Jose, CA, USA Visit us at www.preciaenome.com/microfluidic-droplet-aenerator

Flow™ Single Cell AnalysisR&D System Open Platform Portable Design Easy Operation w/ Touchscreen Flexible Setup, Programmable • Single cell isolation & PCR amplification • Single cell antibody discovery • Single cell analysis • Cell line development • DNA/RNA sequencing library Biopharmaceutical discovery Drug-resistance studies Double emulsion Enzyme evolution Synthetic biology Catalog # Name System w. iFlow Touch™ controller and -highspeed imaging System w. iFlow Touch™ controller Address: 2176 Ringwood Ave., San Jose, CA, USA Visit us at www.precigenome.com/microfluidic-droplet-generator

Single Cell Encapsulation Efficiency PreciGenome Using 10 pm polystyrene beads as a model for mammal cells, droplet encapsulation follows the Poisson distribution. Data shown is for 55-60 pm droplets; expected value is with one-third of initial bead concentration (chip design dependent.) Single Encapsulation - Single Emulsion Beads Concentration vs. Encapsulation Rates The Poisson distribution also holds for other bead concentrations. As bead concentration decreases, a greater percentage of droplets have no or one encapsulation events. Data shown is for 55-60 pm droplets; expected value is one-third...

Single Cell Encapsulation Efficiency PreciGenome Double Emulsion Encapsulation Droplet encapsulation also follows the Poisson distribution in double emulsion systems. Data shown is for 30 pm inner shell; expected value is with one-half of initial bead concentration (chip design dependent.) Address: 2176 Ringwood Ave., San Jose, CA, USA Visit us at www.preciaenome.com/microfluidic-droplet-aenerator

Application: Drop-Seq Working Principle Water Phase 1 (barcoding beads and lysis buffer) Flow direction (cell suspension) In Drop-Seq, the microfluidic chip allows two water phases to mix before being dispersed in the oil phase. This allows barcoding beads and cells to be captured together in droplets. System Diagram Water Phase 1 Water Phase 2 Flow Sensors (optional) PG-MFC Touch Controller High Speed Imaging System (optional) Address: 2176 Ringwood Ave., San Jose, CA, USA Visit us at www.precigenome.com/microfluidic-droplet-generator