Tabular highlights gives a high level view of how iPRECIO pumps were used/programmed in selected scientific publications in Applications Examples eBook. For more detailed view, refer eBook and publications . In e-book for example, Research Application 1 (RA1) on page 5 & 6 of eBook is on row 1 of Tabular highlights – Chavan et al. The protocol is once a day infusion. RA2 on pages 7 & 8 (row 2 Kroon et al) has a 3 day stop prior to start of infusion of Nicotinic acid with the animal in the metabolic cage all of the time of the infusion protocol. Research Application (RA), Author, doi: Refillability of Pump KVO/RCV How pump programmed? Intermittent Circadian / Continuous or bolus Timed release Adipose tissue-diabetes-drug therapyLipoysis and fatty acid metabolismGPR109A-Niacin-Tachyphylaxis Zucker rat + SMP-200 Pump Neural-stem cells-spinal cord injury rat + SMP-200 Pump Regulatory request, CNS end points (modified Irwin assessment) rat + SMP-200 Pump Cardiac, diastolic dysfunction, heart failure, metabolism dog + SMP-200 Pump Pump programmed to stop for 3 days Nicotinic Acid (NiAc) or saline 5 days of continuous or intermittent (12 hr ON/OFF cycles, infusion on 13:00h) PKA and ATP - solution change every 2 days 1ul/hour RA 4 Mitchell et al. Developments in LifeSciences Vol. 14 No. 4 http://www.abstractsonline.com/Plan/ViewAbstract.aspx?mID=2964&sKey=87d8b951-316f-466a-9eb74b154d0bbd2c&cKey=b4b8338f-9bd2-44e2-bcf4-6e05a36cbbcb&mKey=%7b70007181-01C9-4DE9-A0A2EEBFA14CD9F1%7d 1ul/hr saline for 1 week Test articles infusing at 30ul/hr for 72 hours after 1ul/hr saline recovery after catherization for full recovery and pump implantation Pumps programmed to infuse saline (2ul/hr) or ßOHB (2ul/hr) or sodium pyruvate (5ul/hr) or coconut oil (5ul/hr) prior to meal time (6h, ZT22-ZT) in Restricted Food (RF) Biological sciences-NeuroscienceBiochemistry Mice + SMP-300 iPRECIO Pump Adminstration Site 5 or 11 days infusion + 2 days recovery pgs 11-12 of eBook RA 5 Asemu et al. DOI: 10.1152/ajpheart.00674.2012 pg 13 of eBook Author, doi: GLP Studies with iPRECIO Pumps (page 15 eBook) Patten et al pg 15 of eBook Perron et al pg 15 of eBook Saline when not infusing test article Aldosterone (15% ethanol 50% DMSO and 35% water) was continuous infused Glioblastoma, Auger-electron therapy, 125 Brain infusion convection delivery, [ I}5-Iodo-2'deoxyuridine, temozolomide, SMP-200 Pump Treament period 30ul/hour for 72 hours Flow-rate and dosing validation studies on the bench 2 hour baseline, 2 hour infusion (Milrinone or vehicle) right jugular vein Infusion pump, implantation, rat, iPRECIO, Physiological condition SMP-200 pump drug delivery, minipump, osmotic pumps, implantable pump, dose response, quantitative pharmacology, preclinical, telemetry rat + SMP-200 pump drug delivery, minipump, osmotic pumps, implantable pump, dose response, quantitative pharmacology, preclinical, telemetry rat + SMP-200 pump isotone saline, 0.1 mM MTX, then 0.3µg/ml I-UdR was continuously infused CNS (modified Irwin assessment) rat + SMP-200 pump GLP validation GLP validation, jacktet, ambulatory, cardiovascular Dog + iPRECIO Dual 3 days for TA < 7 days 24 hours Author, doi: Toxicology with iPRECIO pumps (page 15 eBook) Tsuboi et al. http://doi.org/10.2131/fts.3.1 pg 15 of eBook No but full study saline Saline infusion for 4 weeks and 13 weeks infusion 2 or 2.5ul/hour Application (PA), Author, doi: Application Examples pg 16 of eBook 3 days pump "OFF" (0.2ul/hour) , for control cardiovascular parameters 5-HT dose response with control period 11 day recovery period Saline, dobutamine and verapamil infusions

Research Application (RA), Author, doi: Refillability of Pump KVO/RCV How pump programmed? Intermittent Circadian / Continuous or bolus Timed release 5ul/hour continous infusion of Ang II. Comparison made with Alzet Pump and Havard Sringe pump 0.2 ul/hr continuos infusion. Stability of vigabatrin was tested and confirmed for 5 weeks hypothalamas, cardiovasular, micro intrainjections hypothalamic rat + SMP-200 pump salt-sensitive hypertension; angiotensin II-salt hypertension; Alzet salt-sensitive SC hypertension; administration minipump; iPrecio pump; plasma angiotensin II Bilateral minipump;...

Research Application (RA), Author, doi: Refillability of Pump KVO/RCV An adipokine feedback regulating diurnal food intake rhythms in mice Mouse Update 1 (MU1) Tsang et al. Tsang et al. eLife 2020;9:e55388. DOI: https://doi.org/10.7554/eLife.55388 MU2 Borovinskaya et al. US Patent Application US 2020/0330445A1 After 2 weeks of recovery (MT experiment; Figure 4) or after 6 weeks of HFD (WT HFD experiment; Figure 6), infusion of AdipoRon (1 mg/ml) or vehicle (DMSO/aCSF/PEG-400) was started, either cyclically with infusions starting at ZT13 for 10 hr followed by 14 hr of inactivity or constantly...

Research Application (RA), Author, doi: Refillability of Pump KVO/RCV Vagus nerve stimulation mediates protection from kidney ischemia-reperfusion injury through α7nAChR+ During the recovery splenocytes period, normal saline was continuously MU5 Inoue et al. infused at the rate of 1 https://doi.org/10.1172/JCI83658 μl/h for 4 days. https://www.jci.org/articles/view/83658 How pump programmed? Intermittent Circadian / Continuous or bolus Timed release Adminstration Site The catheter part of the microinfusion pump was inserted into the left external jugular vein via a midcervical incision. The main...