Cell Health & Metabolism Sensitive, fast and reliable assays Quantifying cell viability and metabolic status is crucial for understanding cancer biology, compound toxicity and cellular responses to stress and other stimuli. Cell Health and Metabolism 2016 - global.indd 1

Cell health and metabolism assays Selecting the most suitable assay to measure cell viability or cytotoxicity from the many options available requires an understanding of the endpoint measured by each assay, how that correlates to cell viability and the specifications of the assay chemistry. Promega provides assays to measure a variety of different markers that indicate the number of live cells, dead cells and the total number of cells or the mechanism of cell death. Biomarkers that can be quantified include ATP, NADH, caspases, LDH and live- and dead-cell proteases. Cell Health and Metabolism...

Assay guide Cell viability assays There is a wide range of viability assays to choose from depending upon, for example, detection instrumentation available. CellTiter-Glo' Luminescent Cell Viability Assay CellTiter-Glo determines the number of viable cells in culture based on quantification of the ATP present and is sensitive to fewer than 10 cells with up to 5-logs of linear range. Simply add the reagent directly to cells in culture, mix and read on a luminometer after 10 minutes incubation. CellTiter-Glo accurately measures cells at numbers below the detection limits of standard colourimetric...

Four versions of CellTiter-Glo CellTiter-Glo® Luminescent Cell Viability Assay Lyophilised substrate and lysis buffer are mixed to produce a reagent with very stable signal (half-life generally >5 hours, dependent on cell type and medium used) allowing batch processing and delivering excellent Z'-factor values for screening applications. RealTime-Glo® MT Cell Viability Assay Analyse potency versus efficacy over time and differential cell growth with a simple, plate-based protocol. NanoLuc® luciferase and a cell-permeant pro-substrate are added to cells. Viable cells reduce the pro-substrate generating...

Cytotoxicity assays Promega offers multiple readouts for quantifying cytotoxicity, including luminescent and fluorescent assays that can be multiplexed with apoptosis or viability assays to help determine the mechanism of cell death. CytoTox-Glo™ Assay Loss of membrane integrity is a key indicator of cytotoxicity to determine necrosis. This is the most sensitive assay to quantify cytotoxicity due to loss of membrane integrity, measuring dead-cell protease biomarker leaked from cytoplasm into medium. Detect as few as 200-500 dead cells in a total population of 10,000 cells. CytoTox-Glo Assay detects...

Multiple measurements in one well Assay Type **please see catalogue for larger pack sizes Assay Type Multiplex assays make it possible to analyse more than one parameter in a sample, simplifying interpretation of the data and enabling differentiation between necrosis and apoptosis, for example. ApoTox-Glo™ Triplex Assay Predictive mechanism of toxicity determination. Measure viability, cytotoxicity and apoptosis in the same sample well to accurately determine the mechanism of cell death, saving time and using fewer cells. Easy to implement in a simple sequential "add-mix-measure" protocol. Normalise...

UGT Activity Assays UGT-Glo™ Assay provides a luminescent method for measuring UDP glucuronosyltransferase (UGT) activity from a variety of sources, such as microsomes containing recombinantly expressed enzymes or microsomal preparations derived from mammalian tissues. The simple "add and read" luminescent format is amenable to higher throughput screening in multiwell plates and eliminates the need for time-consuming analyses such as HPLC and LC/MS. UGT-Glo™ Screening Systems contain the same core assay reagents as well as the respective UGT isoforms (UGT1A1 or UGT 2B7) and control membranes....

Glucose uptake assay Oxidative stress assays Glucose metabolism is a key process in many organisms; lack of insulin-stimulated glucose uptake is associated with type 2 diabetes; high glucose uptake is a sign of the high glycolytic rates associated with cancer. Measuring glucose uptake can determine the effects of various treatments for diabetes and cancer. Glucose Uptake-Glo™ demonstrates the same level of sensitivity as the radioactive glucose uptake assay. It is a simple, plate-based, add-mix-measure, homogeneous, bioluminescent assay, based on the detection of 2-deoxyglucose-6-phosphate (2DG6P)....

GloMax® Discover and Explorer Systems The highest sensitivity, broadest dynamic range, and best value for researchers! • Plug-n-play assay set-up • Preloaded protocols • Integrated with Promega assays • Automation-friendly for your workflow • indows® 8 Tablet PC controlled with state-of W the-art touchscreen control and connectivity • uminescence, fluorescence, UVL Vis absorbance, BRET, FRET, filtered luminescence available • 384 and 96-well plate formats • Heating / shaking / injectors • ntegrated filter paddles for assay I multiplexing • art 11 Electronic Signature p software compliance • SiLA-ready...