CHARACTERIZE CELL SIGNALING IN YOUR SMALLEST SAMPLES

REVOLUTIONIZE PHOSPHOPROTEIN SIGNALING STUDIES ProteinSimple’s NanoPro™ 1000 provides exciting new insights into protein function and cell signaling. Where traditional protein analysis techniques require thousands to millions of cells, the NanoPro 1000 can analyze as few as 25 cells per assay. Direct characterization of signal transduction proteins can now be performed in precious and extremely small samples such as: • Primary cells • Isolated stem cell populations • Fine-needle tumor aspirates • Micro-biopsies • Laser Capture Microdiscection (LCM) Critical details about intracellular control...

REVEAL PREVIOUSLY UNDETECTABLE INFORMATION REFRESH YOUR POINT OF VIEW: NOVEL APPLICATIONS WESTERN BLOT Conventional Western blot results are variable, semi-quantitative, and provide limited isoform identification. ERK1 and ERK2 appear as two poorly resolved bands using Western blot analysis. In contrast, the NanoPro 1000 resolves six bands: dual, mono, and non-phosphorylated isoforms of ERK1 and ERK2. NanoPro 1000 assays uncover fundamental mechanisms controlling cell proliferation and cell death, accelerate the development of new therapeutics, and help identify new prognostic and diagnostic...

TECHNOLOGY: A NEW TAKE ON IMMUNOASSAYS The NanoPro 1000 is a capillary-based nano-immunoassay platform. As in Western blot analysis, proteins from complex biological samples are separated, immobilized, and probed with antibodies. However, the NanoPro 1000 uses isoelectric focusing (IEF) to resolve the various phosphorylation states of signaling proteins. NanoPro 1000 assays are fully automated, and all steps are performed in a single capillary. A precision robot moves capillaries to the sample, reagent, incubation, and separation chambers as dictated by the assay protocol. Chemiluminescent image...

ENVISION DATA THAT PROVIDE NEW INSIGHTS The NanoPro 1000 generates much more precise and accurate data than traditional techniques, and its Compass software translates those data into easily understandable, useful results. • Isoform percent totals for each protein are automatically calculated • Data can be viewed in three formats: graph, digital capillary photo or virtual gel-like image • IEF separations are recorded — videos can be viewed at any time • Results tables and data views can be easily copied into reports and presentations Proteins are automatically identified by name or pI COMPASS...

SPECIFICATIONS AND ORDERING INFORMATION ASSAY SPECIFICATIONS DESCRIPTION IMMUNOASSAY CHARGE SPECIFICATION Sample Required Volume Required Widest gradient ranges from pI 3 to pI 10 Resolution (± percent difference in MW) Dynamic Range * Percent peak area SYSTEM SPECIFICATIONS SPECIFICATION Power Requirements Power Consumption Operating Humidity Range Operating Temperature Range Sample Cooling

ORDERING INFORMATION PART # NanoPro 1000-Includes instrument, computer, Windows operating system, Compass software (10-seat license), and one year warranty NanoPro1000 IQ/OQ Validation Service Authorization Server. Used in conjunction with the Compass Software Access Control feature (10-seat license). Supports Compass Software 21 CFR Part 11 data security requirements when using Simple Western instruments

WHERE SCIENCE INTERSECTS INNOVATION ™ At ProteinSimple, we’re changing the way scientists analyze proteins. Our innovative product portfolio helps researchers reveal new insight into proteins, advancing their understanding of protein function. We enable cutting-edge research to uncover the role of proteins in disease and provide novel approaches to develop and analyze protein-based therapeutics. We empower you to make your next discovery by eliminating common protein analysis workflow challenges. For more information visit or contact us at: Toll-free: 888 607 9692 Tel: 408 510 5500 [email protected]...