YOUR COMPLETE PROTEIN ANALYSIS SOLUTION
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YOUR COMPLETE PROTEIN ANALYSIS SOLUTION

MEET JESS. Protein analysis comes with many challenges—labor-intensive protocols increase time to result and multiple hands-on steps increase user error and data variability. At best, you end up with Western blot-like, semi-quantitative results when what you really need, and deserve, is highly reproducible immunoassay quantitation. Meet Jess, your protein analysis problem solver. Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. Just load your samples and reagents into the microplate, and Jess does...

HIGH THROUGHPUT Jess precisely controls sample loading, incubations and washes; she eliminates the inconsistencies and user-dependent variability that can be introduced during traditional Westerns. She delivers intra-assay CVs <15%, giving you the consistency you need to be confident in your data. With her 13 and 25 sample capillary cartridges, you’ll get the throughput you need, with minimum hands-on time. Use Jess’s fluorescent capabilities and multiplex your proteins for even higher throughput. With Jess, it’s pipette, run and done! Simply load your sample, antibodies and reagents into...

WHY SETTLE? DO PROTEIN ANALYSIS YOUR WAY, QUICKLY. JESS GIVES YOU FOUR DIFFERENT WAYS TO ANALYZE PROTEINS. JESS WITH CHEMILUMINESCENCE JESS WITH STELLAR NIR JESS WITH STELLAR IR COMPETING FLUORESCENCE WESTERN BLOTTING TECHNOLOGY Jess with Chemiluminescence Jess with Stellar NIR / IR Why bother stripping and reprobing? Maximize your time and sample using Stellar NIR/IR modules for market-leading fluorescent detection sensitivity. 2 CHEMILUMINESCENCE DETECTION RNase A Working with low abundance targets or precious samples? Chemiluminescent detection gives you picogram-level sensitivity,...

STOP, ANALYZE AND WOW! At the end of your run, use the lane view option to compare band intensity or dive deep for fully quantitative analysis of Protein Size and concentration. Dive deeper to compare protein expression changes and analyze protein isoforms or size changes. Want to analyze expression changes between samples or compare runs? Jess’s protein normalization will give you the confidence you need in your analysis. AUTOMATED IMMUNOASSAYS AND TOTAL PROTEIN NORMALIZATION EQUALS BETTER QUANTIFICATION Pan AKT Probe 2: Total Protein Total Protein Immunoassay and total protein detection...

HOW DOES JESS WORK? FLUORESCENT DETECTION CHEMILUMINESCENT DETECTION Load Matrix Load Matrix Stacking Matrix Stacking Matrix Separation Matrix Separation Matrix Load Sample Load Sample Target Protein 2 Primary Ab Quantitate Signal Target Protein 2 Primary Ab Target Protein Primary Ab Quantitate Signal FLUORESCENT PROTEIN NORMALIZATION Load Matrix and Sample Load Matrix Stacking Matrix REPLEX™ WITH JESS™ Separation Matrix Load Sample Separate & Immobilize Detect and Quantitate Target 1 Quantitate Signal RePlex (remove antibody from 1st Immunoprobe) Protein Normalization Reagent Antibody 2...

TOTAL PROTEIN SPECIFICATION CHEMILUMINESCENCE SPECIFICATION FLUORESCENCE SPECIFICATION PROTEIN NORMALIZATION SPECIFICATION Sample required Volume required Size range Molecular weight (MW) ladder ranges from 2–440 kDa Resolution (± percent difference in MW) <20% (total protein, chemiluminescence and fluorescence) Dynamic range <3 hours RePlex: 5 hours Dimensions (closed) Dimensions (open) US/CAN 120 V AC, 60 Hz, 4.2 amps Europe 240 AC, 50 Hz, 2.1 amps Japan 100 AC, 50/60 Hz, 5.0 amps Operating temperature Operating humidity <4 hours with immunoassay * Inter-assay CV is with system c

WHERE SCIENCE INTERSECTS INNOVATION ™ At ProteinSimple, we’re changing the way scientists analyze proteins. Our innovative product portfolio helps researchers reveal new insight into proteins, advancing their understanding of protein function. We enable cutting-edge research to uncover the role of proteins in disease and provide novel approaches to develop and analyze protein-based therapeutics. We empower you to make your next discovery by eliminating common protein analysis workflow challenges. For more information visit or contact us at: Toll-free: 888 607 9692 Tel: 408 510 5500...