PAXgene Blood miRNA Kit Brochure
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For purification of total RNA, including microRNA, from whole blood stabilized in PAXgene Blood RNA Tubes The PAXgene Blood miRNA System* consists of PAXgene Blood RNA Tubes for collection, stabilization, and transport of blood, and the PAXgene Blood miRNA Kit for purification of total RNA, including RNA longer than approximately 18 nucleotides. Purification is carried out using silica-based RNA purification technology in a spin-column format. Purification can be carried out manually, using a microcentrifuge, or automated on the QIAcube® (Figure 1). Used with the tubes, the kit provides a complete preanalytical solution for collection and stabilization through to purification of high-quality total RNA, including miRNA, for molecular analysis. Figure 1. The QIAcube. Samples can be processed manually or automated on the QIAcube. Benefits of the PAXgene Blood miRNA System include: I Integrated system for collection, stabilization, and purification RNA stabilization for up to 3 days at 18–25°C or at least 50 months at –20°C or –70°C * For Research Use Only. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. Effective purification of total RNA, including miRNA

least 50 months without showing any significant RNA degradation or changes in transcript levels. Integrated system for collection, stabilization, and purification Blood samples are collected in PAXgene Blood RNA Tubes, which contain a reagent that lyses blood cells and immediately stabilizes intracellular RNA to preserve the gene expression profile. RNA stabilization is critical for reliable downstream gene expression analysis. Without stabilization, degradation of RNA and upregulation or downregulation of transcripts occur immediately after blood is drawn. Blood samples collected in...

High-quality RNA and miRNA for a wide range of applications The purified total RNA is ready to use and is ideally suited for any downstream application, including: cDNA synthesis Northern, dot, and slot blot analyses Primer extension Poly A+ RNA selection RNase/S1 nuclease protection Microarray analysis RT-PCR and real-time RT-PCR Differential display miScript Primer Assay hsa-mir 16 27 miScript Primer Assay hsa-mir 30b 27 PAXgene Blood miRNA Kit PAXgene Blood RNA Kit PAXgene Blood miRNA Kit PAXgene Blood RNA Kit miScript Primer Assay hsa-mir 192 39 miScript Primer Assay hsa-mir 10a 39...

Ordering Information Product PAXgene Blood miRNA Kit (50) 50 PAXgene Spin Columns, Processing Tubes, 763134 RNase-Free DNase I, RNase-Free Reagents and Buffers. To be used in conjunction with the PAXgene Blood RNA Tubes PAXgene Blood RNA Tubes (100) 100 Blood Collection Tubes; available from BD 762165 For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor. Trademarks:...