RNeasy® Mini, Midi and Maxi Kits
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RNeasy® Mini, Midi and Maxi Kits For purification of total RNA from cells, tissues, and yeast, and for RNA cleanup* RNeasy Mini, Midi and Maxi Kits provide a fast and convenient procedure for RNA purification, delivering high-quality RNA for use in many downstream applications. RNA is isolated on the silica membrane in trusted RNeasy spin columns, which have binding capacities of 100 µg (mini), 1 mg (midi) and 6 mg (maxi) RNA. Benefits of RNeasy Mini, Midi and Maxi Kits: RNeasy Procedure • Fast procedure delivering high-quality total RNA in minutes • Ready-to-use RNA for use in many downstream applications • Consistent RNA yields from small to large samples • No phenol/chloroform extraction or LiCl or ethanol precipitation Lyse, homogenize, and add ethanol Fast, reproducible RNA purification Bind total RNA to RNeasy membrane RNA purification with the RNeasy Mini Kit takes just 20 minutes, and can be performed using a microcentrifuge or QIAvac vacuum manifold or fully automated on the QIAcube®. With RNeasy Midi and Maxi Kits, RNA is purified in less than 1 hour using a centrifuge. All kits deliver reproducible yields of intact RNA with high RIN values and A260/A280 ratios of 1.9–2.1 (Figures 1 and 3). Safe and convenient analysis of RNA integrity can be achieved using the QIAxcel® system (Figure 3A), which analyzes up to 96 samples on ready-to-use gel cartridges. Elute in small volume Figure 1. Reproducible RNA yields with RNeasy Kits. RNA was purified from eight HeLa cell samples (106 cells each) using either the RNeasy Mini Kit (RNeasy) or acid-phenol–extraction (Acid Phenol). A UV spectrophotometry of samples diluted in 10 mM Tris·Cl, pH 7.5. B Formaldehyde agarose gel analysis of 10 μg of each sample, based on A260 readings in A. * For difficult-to-lyse tissues, specialized RNeasy Kits are available. For details, visit www.qiagen.com/RNA. Figure 2. High RNA yields from various tissues. Rat tissues (15 mg) were stabilized in RNAlater® RNA Stabilization Reagent and disrupted using the TissueLyser II. RNA was purified using the RNeasy Mini Kit, either manually (Manual) or on the Q

High performance in downstream applications QIAGEN® QIAxcel Fluorescence Ready-to-use RNA can be purified from a variety of sample types using RNeasy Kits, and yields are comparable when purification is either carried out manually or automated on the QIAcube (Figure 2). The innovative QIAcube automates the binding, washing, and elution of RNA, and can process up to 12 samples per run. RNA purified using RNeasy Kits is longer than 200 nucleotides and performs well in a wide range 0 Agilent Bioanalyzer of applications, including RT-PCR and real-time RT-PCR (Figure 4). CT 40 GAPDH (HEX) RPS27A...