Rotor-Gene Q - Pure Detection
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Now with even more applications! •••••QIAGEN Sample & Assay Technologies

The Rotor-Gene Q — for your success Quantitative, real-time PCR is a precision science that places high demands on the instrument, chemistry, and software. High thermal and optical uniformity, short equilibration times, and fast ramping rates are critical for precise and rapid quantitative analysis. Sensitivity, speed, and specificity are also highly dependent on the performance of the DNA polymerase and reaction components. QIAGEN's real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to provide the outstanding performance and reliable results that your...

Unique rotary design for outstanding performance The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available (Figure 1). Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform. When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. This thermal and optical uniformity results in sensitive, precise, and fast...

Unrivaled optical range enables multiple applications Whether your assay is based on intercalating dyes such as SYBR® Green, probes such as hydrolysis (TaqMan), hybridization (FRET), or Scorpion probes, the Rotor-Gene Q meets your requirements. With up to 6 channels spanning UV to infrared wavelengths, the cycler delivers the widest optical range currently available (Table 1), and is highly suited for all multiplex applications. In addition, the software allows you to create new excitation/detection wavelength combinations, which means that the Rotor-Gene Q is compatible with dyes you may...

Easy routine verification Laboratories may often want to verify thermal accuracy. For most cyclers, this requires interaction with a service engineer. With the Rotor-Gene Q, this is not necessary. Instead, the easy-to-use, cost-effective Rotor-Disc OTV (Optical Temperature Verification) Kit automates accuracy testing. The kit includes a specialized Rotor-Disc filled with temperature-sensitive liquid crystals and dedicated analysis software. The full procedure takes less than 30 minutes. Reliable support for your peace of mind In the unlikely event of any service issues with your Rotor-Gene...

Software enables quantification and enhances data security The comprehensive Rotor-Gene Q software package supports all current state-of-the-art real-time analysis procedures with basic to advanced algorithms. This provides complete freedom to analyze your valuable experimental data and increases the reliability of your results. Data security is assured and process steps are trackable from starting the run to exporting the results. Analysis procedures supported by Rotor-Gene Q software Absolute quantification Analysis of genetic differences Standard curve quantification Allelic...

Expand your research with HRM Normalized fluorescence Normalized fluorescence High-resolution melting analysis (HRM) is a closed-tube, post-PCR analysis that has raised enormous scientific interest. HRM characterizes double-stranded PCR products based on their dissociation (melting) behavior. It is similar to classical melting curve analysis, but provides far more information for a wider range of applications. PCR products can be discriminated according to sequence, length, GC content, or strand Normalized minus WT WT Normalized minus characterized in a robust and straightforward way. The...

Optimized reagents for all your applications A range of QIAGEN kits for the Rotor-Gene Q enables reliable quantification in your real-time PCR applications without the need for optimization of reaction and cycling conditions (Table 3). Features and benefits of QIAGEN's kits for real-time PCR Highly specific amplification is assured through a balanced combination of NH4+ and K+ ions that minimizes nonspecific primer annealing. Fast cycling without compromising performance is achieved using Q-Bond, a proprietary PCR additive that enables cycler run times of as low as 45 minutes. For multiplex...

Gene expression analysis Amplification plot (QIAGEN) Assays, and the Rotor-Gene Q provides a complete, ready-to-run solution for gene expression analysis. QuantiTect Primer Assays are predesigned primer sets for any gene from human, mouse, rat, and many other species. Assays can be easily ordered online at the GeneGlobe® Web portal (www.qiagen.com/GeneGlobe). When the assays are used together with Rotor-Gene SYBR Green Kits, highly sensitive quantification of specific PCR products is achieved without the need for optimization (Figure 5 and Table 4). B Normalized fluorescence The combination...

The ultimate solution for fast and reliable gene expression ana lysis is provided by the combination of the Rotor-Gene Multiplex PCR and RT-PCR Kits and the Rotor-G ene Q. Up to 4 cDNA targets can be simultaneously and rapidly quantified in the same tube, increasing throughput and saving precious sample material (Figures 7 and 8). Genes with different expression levels are all amplified in the same tube with the same high efficiency, enabling reliable relative quantification of gene expression. Figure 7. Reliable multiplex analysis. Tenfold dilutions of human leukocyte cDNA (10 ng to 10 pg)...

Pathway- and disease-focused PCR arrays Pathway-focused RT2 Profiler PCR Arrays are exeptionally reliable tools for analyzing the expression of a focused panel of genes. Each 100-well Rotor-Disc PCR Array includes SYBR Green-optimized (laboratory-tested) primer assays for a thoroughly researched panel of relevant, pathway- or disease-focused genes on the Rotor-Gene Q. RT2 profiler PCR Arrays can also be customized to contain a panel of genes tailored to your specific research interests. Our high-quality primer design and master mix formulation enable the RT2 profiler PCR Array to amplify 96...