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Q-Plex™ Bovine Cytokine Panel 1 (5-Plex)
Q-Plex™ Bovine Cytokine Panel 1 (5-Plex)
The Q-Plex™ Bovine Cytokine Panel 1 (5-Plex) is a quantitative multiplex ELISA designed for detecting cytokines related to inflammation in bovine samples. It is a plate-based assay that utilizes a 96-well solid plate format and chemiluminescent detection.
Specifications
- Assay Type: Sandwich
- Sample Types: Serum, plasma, and cell culture supernatant
- Assay Length: 3.5 hours
- Volume Required Per Well: Minimum 25 µL
- Species Specificity: Bovine
- Catalog Number: 112349BV
Performance and Validation Data
The panel measures five cytokines: IFNγ, IL-4, IL-10, IL-12p40, and TNFα. Each cytokine has specific calibrator ranges, limits of quantification, and detection limits. The assay demonstrates high precision with inter-assay and intra-assay variability ranging from 3% to 14%.
Key Parameters
- IFNγ: Calibrator range 1,650 - 2.94 pg/mL, ULOQ 1,155 pg/mL, LLOQ 2.94 pg/mL, detection limit 1.47 pg/mL, inter-assay precision 14%, intra-assay precision 5%, linearity 109.64%.
- IL-4: Calibrator range 500 - 0.89 pg/mL, ULOQ 350 pg/mL, LLOQ 0.89 pg/mL, detection limit 0.45 pg/mL, inter-assay precision 12%, intra-assay precision 3%, linearity 106.97%.
- IL-10: Calibrator range 5,000 - 8.92 pg/mL, ULOQ 1,166 pg/mL, LLOQ 8.92 pg/mL, detection limit 4.46 pg/mL, inter-assay precision 10%, intra-assay precision 3%, linearity 108.37%.
- IL-12p40: Calibrator range 200,000 - 365 pg/mL, ULOQ 140,000 pg/mL, LLOQ 365 pg/mL, detection limit 178 pg/mL, inter-assay precision 12%, intra-assay precision 7%, linearity 99.93%.
- TNFα: Calibrator range 3,000 - 16.05 pg/mL, ULOQ 2,100 pg/mL, LLOQ 16.05 pg/mL, detection limit 8.03 pg/mL, inter-assay precision 13%, intra-assay precision 4%, linearity 114.68%.
Company Information
Quansys Biosciences is ISO 9001:2015 and ISO 13485:2016 certified, ensuring compliance with GMP. The company is based in Logan, UT, USA.
Catalog excerpts
Q-Plex™ Bovine Cytokine Panel 1 (5-Plex) The Q-Plex™ Bovine Cytokine Panel 1 (5-Plex) is a fully quantitative plate-based multiplex ELISA for the detection of cytokines associated with inflammation
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Background The Q-Plex™ Bovine Cytokine Panel 1 (5-Plex) is a fully quantitative plate-based multiplex ELISA for the detection of cytokines associated with inflammation. Production of inflammatory cytokines occurs normally to pro tect the host from pathogens, pain and wound healing, and growth signaling. However, excessive, or chronic production of inflammatory cytokines can damage host tissues and is associated with certain autoimmune diseases and cancers. Volume Required Per Well Min. 25 pL Detection Mention Chemiluminescent Multiplex Format 96-well solid plate Species Specificity Bovine Assay...
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