Q-Plex™ Human Angiogenesis (9-Plex)

Product catalog summary

Overview: The document provides detailed information about the Q-Plex™ Human Angiogenesis (9-Plex) assay, a quantitative ELISA-based chemiluminescent assay designed for the concurrent measurement of nine biomarkers: ANG-2, FGF basic, HGF, IL-8, PDGF-BB, TIMP-1, TIMP-2, TNFα, and VEGF.

Specifications: Each kit includes a 96-well plate with capture antibodies arranged in a defined array, allowing for the measurement of up to 80 samples using only 50 µL of sample per well. The assay is completed within 2.5 hours.

Assay Performance: The document lists the calibrator range, upper and lower limits of quantification, limit of detection, and precision (both inter-assay and intra-assay) for each analyte. For example, ANG-2 has a calibrator range of 10,000 – 13.72 pg/mL, with an upper limit of quantification of 7000 pg/mL and a lower limit of 17.8 pg/mL.

Procedure: The assay involves adding a sample, washing, adding detection antibodies, washing again, and then adding streptavidin conjugated HRP. The presence of antigen is detected by a signal emitted from the spot, proportional to the antigen amount.

Data Analysis: Image analysis is performed using Q-View™ Software, which automatically identifies spots and measures intensity. Data is analyzed against in-plate standards, and regression models are used to calculate unknowns.

Product Features: The Q-Plex™ Arrays are described as cost-effective and user-friendly, with the Q-View™ Imager LS providing high-quality imaging for chemiluminescent assays. The software facilitates the acquisition and analysis of multiplex ELISA data.

Quality and Compliance: Quansys Biosciences is ISO 9001:2015 and ISO 13485:2016 registered, ensuring compliance with GMP. Products are developed according to a Quality Management System.

Contact Information: Quansys Biosciences is located in Logan, UT, with contact details provided for further inquiries.

Catalog excerpts

The Q-Plex Human Angiogenesis (9-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of nine biomarkers or analytes: ANG-2, FGF basic, HGF, IL-8, PDGF-BB, TIMP-1, TIMP-2, TNFa, and VEGF. Each kit contains a 96-well plate, featuring the relevant biomarker panel, and all the reagents required to perform testing. Q-Plex™ plates are built by absorbing nine distinct capture antibodies in a defined array to the bottom of each well. Our high quality reagents help ensure the accuracy of your results. Using just 50 pL of sample per well, up to 80 samples can be assayed for all nine markers in the panel within 2.5 hours. Q-Plex™ Arrays provide researchers with an easy-to-use and cost effective means of generating a cytokine profile for each sample. Catalog # Product 150249HU Q-Plex™ Human Angiogenesis (9-plex) Product Range Analyte ‘ Actual values may vary from kit to kit. Please see the In-Kit Certificate of Analysis included in your kit for specific values.

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Increase productivity with Quansys products and services. Save time, sample, and money. Spotting in 96 Well Plate ► Robotic liquid handlers print 20-50nL spots of capture antibody ► Each spot is a unique assay within the well ► Low spot-to-spot variability (CV) ► Spot size 350-500pm ► Plates are QC'd for spot quality Q-Plex" ARRAYS Performing the Assay ► Add as little as 25pL of sample ► Wash ► Add mix of detection antibodies specific to kit ► Wash ► Add streptavidin conjugated HRP Our Q-Plex Arrays are quantitative multiplex ELISAs with distinct proteins deposited in a defined array. Choose...

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