Q-Plex™ Mouse Cytokine Screen HS (15-Plex)

Product catalog summary

Overview: The Q-Plex™ Mouse Cytokine Screen HS (15-Plex) is a quantitative ELISA-based chemiluminescent assay designed for the simultaneous measurement of 16 biomarkers, including GMCSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17A, MIP-1α, TNFα, and RANTES. Each kit includes a 96-well plate with the necessary reagents for testing.

Specifications: The assay uses a sandwich format with specific calibrator ranges, upper and lower limits of quantification, and detection limits for each analyte. Precision is measured both inter-assay and intra-assay, with average linearity provided for each biomarker.

Procedure: The assay involves adding a small sample volume, washing, adding detection antibodies, washing again, and adding streptavidin conjugated HRP. The presence of antigen is detected by a chemiluminescent signal.

Image and Data Analysis: The Q-View™ Imager LS captures high-resolution images of the plate, which are analyzed using Q-View™ Software. The software automatically identifies spots, measures intensity, and generates raw data. Data analysis involves comparing raw data to in-plate standards and using regression models to calculate unknowns.

Product Features: The Q-Plex Arrays are customizable multiplex ELISAs with distinct proteins in a defined array. The Q-View Software facilitates the acquisition and analysis of multiplex ELISA data, enhancing productivity and efficiency.

Quality and Compliance: Quansys Biosciences is ISO 9001:2015 and ISO 13485:2016 registered, ensuring compliance with GMP. Products are developed under a robust Quality Management System.

Contact Information: Quansys Biosciences, 365 North 600 West, Logan, UT 84321. Phone: 435-752-0531, Toll-Free: 888-QUANSYS (782-6797).

Catalog excerpts

Q-Plex™ Mouse Cytokine Screen HS (15-Plex)-1

Q-Plex™ Arrays Q-Plex™ Mouse Cytokine Screen HS (15-Plex) The Q-Plex Mouse Cytokine Screen HS (15-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of sixteen biomarkers or analytes: GMCSF, IFNy, IL-1a, IL-1P, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17A, MIP-1a, TNFa, RANTES. Each kit contains a 96-well plate, featuring the relevant biomarker panel, and all the reagents required to perform testing. Q-Plex™ plates are built by absorbing sixteen distinct capture antibodies in a defined array to the bottom of each well. Our high quality reagents help ensure the accuracy of your results. 112449MS-15P Q-Plex Mouse Cytokine Screen HS (15-Plex) Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Precision1 Average1 (Intra- Linearity assay) GMCSF Sandwich 560 - 0.77 (pg/mL) Actual values may vary from kit to kit. Please see the In-Kit Certificate of Analysis included in your kit for specific values.

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Q-Plex™ Mouse Cytokine Screen HS (15-Plex)-2

Increase productivity with Quansys products and services. Save time, sample, and money. Spotting in 96 Well Plate ► Robotic liquid handlers print 20-50nL spots of capture antibody ► Each spot is a unique assay within the well ► Low spot-to-spot variability (CV) ► Spot size 350-500pm ► Plates are QC'd for spot quality Q-Plex ARRAYS Performing the Assay ► Add as little as 25pL of sample ► Wash ► Add mix of detection antibodies specific to kit ► Wash ► Add streptavidin conjugated HRP Our Q-Plex Arrays are quantitative multiplex ELISAs with distinct proteins deposited in a defined array. Choose one...

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