OverviewThe Q-Plex NHP Cytokine Screen (16-Plex) is a quantitative ELISA-based chemiluminescent assay designed for the simultaneous measurement of 16 biomarkers. It is developed by Quansys Biosciences, a company compliant with ISO 9001:2015 and ISO 13485:2016 standards.
Specifications-
Assay Type: Sandwich
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Sample Type: Serum, Plasma
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Assay Length: 4 hours
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Volume Required Per Well: Minimum 25 µL
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Detection Method: Chemiluminescent
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Multiplex Format: 96-well solid plate
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Catalog Number: 110933MB
Performance and ValidationThe assay uses 16 distinct capture antibodies arranged in a defined array on a 96-well plate, allowing for the measurement of up to 80 samples for all 16 markers within 4 hours. The assay ensures high accuracy and precision with minimal sample volume.
Key Analytes and Performance Metrics- GCSF: ULOQ: 3,150 pg/mL, LLOQ: 8.02 pg/mL, Precision: Inter-assay 14%, Intra-assay 7%
- GMCSF: ULOQ: 4,200 pg/mL, LLOQ: 10.7 pg/mL, Precision: Inter-assay 8%, Intra-assay 8%
- IFNγ: ULOQ: 700 pg/mL, LLOQ: 1.78 pg/mL, Precision: Inter-assay 8%, Intra-assay 5%
- IL-1β: ULOQ: 2,852.5 pg/mL, LLOQ: 7.27 pg/mL, Precision: Inter-assay 9%, Intra-assay 7%
- IL-2: ULOQ: 455 pg/mL, LLOQ: 1.16 pg/mL, Precision: Inter-assay 7%, Intra-assay 9%
- IL-4: ULOQ: 42 pg/mL, LLOQ: 0.11 pg/mL, Precision: Inter-assay 16%, Intra-assay 12%
- IL-5: ULOQ: 233.1 pg/mL, LLOQ: 0.59 pg/mL, Precision: Inter-assay 17%, Intra-assay 9%
- IL-6: ULOQ: 700 pg/mL, LLOQ: 1.78 pg/mL, Precision: Inter-assay 16%, Intra-assay 6%
- IL-8: ULOQ: 1,400 pg/mL, LLOQ: 3.57 pg/mL, Precision: Inter-assay 12%, Intra-assay 6%
- IL-10: ULOQ: 1,867 pg/mL, LLOQ: 4.78 pg/mL, Precision: Inter-assay 13%, Intra-assay 4%
- IL-12p70: ULOQ: 2,100 pg/mL, LLOQ: 5.35 pg/mL, Precision: Inter-assay 16%, Intra-assay 12%
- IL-13: ULOQ: 233.1 pg/mL, LLOQ: 0.59 pg/mL, Precision: Inter-assay 11%, Intra-assay 8%
- IL-15: ULOQ: 2,504.6 pg/mL, LLOQ: 6.38 pg/mL, Precision: Inter-assay 18%, Intra-assay 7%
- IL-17a: ULOQ: 3,912.3 pg/mL, LLOQ: 9.97 pg/mL, Precision: Inter-assay 19%, Intra-assay 13%
- IL-18: ULOQ: 151 pg/mL, LLOQ: 0.38 pg/mL, Precision: Inter-assay 13%, Intra-assay 4%
- TNFα: ULOQ: 175 pg/mL, LLOQ: 0.45 pg/mL, Precision: Inter-assay 11%, Intra-assay 3%
Contact InformationQuansys Biosciences, 365 North 600 West, Logan, UT 84321
Website:
www.quansysbio.comPhone: 435-752-0531, Toll-Free: 888-QUANSYS (782-6797)