PerfeCTa SYBR Green SuperMix/FastMix ® Sensitive and precise DNA amplification with SYBR-based quantification FEATURES & BENEFITS: concentrated reagents minimize pipetting steps, simplify reaction assembly and improve accuracy 2x Superior assay sensitivity and specificity with ultrapure AccuStart enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot start Supports efficient vortex mixing with proprietary anti-foaming formulation FastMix formulation supports both fast and standard thermal cycling conditions uperMix version provides maximum dye concentration for robust optical signal with small amplicons S (i.e. microRNAtemplated cDNA) DESCRIPTION: Specific target amplification is essential for precise target quanti- Single-tube reagents are 2x concentrated ready-to-use reaction fication with SYBR Green I technology since this dye binds cocktails containing all necessary components, except primers to all dsDNA generated during amplification. PerfeCTa SYBR and DNA template for quantitative PCR. Proprietary formulation Green SuperMix and FastMix ensure specific primer extension stabilizes SYBR Green I dye to deliver maximum efficiency, sensi- products with ultra-pure AccuStart hot start technology and tivity, and robust fluorescent signal. Store refrigerated (4°C) proprietary formulation that reduces potential for primer-dimer for up to 6 months, protected from light or ≤–20°C for up to and other non-specific artifacts. one year. Reagent performance is not affected by repetitive freeze-thawing. Cycle Threshold Cycle Threshold 0.01 0.1 1.0 10 Initial Quantity (ng total RNA) 2 3 4 5 Log Initial Quantity (pg HeLa total RNA) Failed qPCR 1 pg cDNA PerfeCTa SYBR Green SuperMix Competitor A PKCA target amplified from log-fold serial dilutions of qScript synthesized cDNA from HeLa cell total RNA (100 ng – 1 pg) with either PerfeCTa SYBR Green SuperMix or Power SYBR Green PCR Master Mix. For more info visit: www.quantabio.com PerfeCTa SYBR Green FastMix iQ SYBR Green Supermix ADAR target amplified from log-fold serial dilutions of qScript synthesized cDNA from HeLa cell total RNA (100 ng – 10 pg) with either PerFeCTa SYBR Green FastMix or iQ SYBR G

Product Name Trademarks: FastMix® and PerfeCTa® are trademarks of QIAGEN Beverly, Inc.; SYBR is a trademark of Life Technologies Corporation. Quantabio products are intended for molecular biology applications. The products are not intended for the diagnosis, prevention or treatment of a disease. MK-SF-0008 REV 01 PerfeCTa SYBR SM_FM 0918 For more info visit: www.quantabio.com