Recombinant Human Activin A

Product catalog summary

Source and Structure
Recombinant Human Activin A protein is derived from Chinese Hamster Ovary (CHO) cells. It is a disulfide-linked homodimer with a predicted molecular mass of 13 kDa. The protein sequence spans Gly311 to Ser426, with an accession number P08476.2.

Specifications
The protein exhibits a molecular mass of 13 kDa under reducing conditions and 24 kDa under non-reducing conditions as determined by SDS-PAGE. Its activity is measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells, with an effective dose (ED50) ranging from 0.200 to 1.60 ng/mL. The endotoxin level is less than 0.01 EU per 1 μg of protein, and purity exceeds 95% as visualized by SDS-PAGE and Coomassie® Blue Staining.

Formulation and Storage
The protein is lyophilized from a solution containing acetonitrile, TFA, and trehalose. For reconstitution, a 20 μg size should be dissolved at 100 μg/mL in sterile 4 mM HCl, while other sizes should be reconstituted at 500 μg/mL. The product is shipped with polar packs and should be stored at -20 to -70 °C for up to 12 months. After reconstitution, it remains stable for 1 month at 2 to 8 °C and for 3 months at -20 to -70 °C.

Bioactivity
Recombinant Human Activin A induces hemoglobin expression in K562 cells, with an ED50 of 0.200-1.60 ng/mL. SDS-PAGE analysis shows bands at 13 kDa and 24 kDa under reducing and non-reducing conditions, respectively.

Background and Applications
Activin A, part of the TGF-β superfamily, plays roles in tissue morphogenesis, fibrosis, inflammation, and more. It forms homodimers or heterodimers with various β subunits. Activin A binds to type 2 serine/threonine kinase receptors, leading to Smad activation and gene transcription regulation. Its bioactivity is modulated by molecules like BAMBI and Follistatin. Activin A is crucial in cell differentiation, immune evasion by tumors, and regenerative medicine applications.

References
The document cites numerous studies and articles that explore the roles and mechanisms of Activin A in various biological processes and therapeutic applications.

Catalog excerpts

biotechne RsDsystems Recombinant Human Activin A (CHO derived) Catalog Number: 11348-ACDESCRIPTION Source Chinese Hamster Ovary cell line, CHO-derived human Activin A protein Gly311-Ser426 Accession # P08476.2 N-terminal Sequence Gly311 Analysis Structure / Form Disulfide-linked homodimer Predicted Molecular 13 kDa Mass SPECIFICATIONS SDS-PAGE 13 kDa under reducing conditions and 24 kDa under non-reducing conditions. Activity Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.200-1.60 ng/mL. Endotoxin Level <0.01 EU per 1 gg of the protein by the LAL method. Purity >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. Formulation Lyophilized from a 0.2 gm filtered solution in Acetonitrile and TFA with Trehalose. See Certificate of Analysis for details. PREPARATION AND STORAGE Reconstitution Reconstitute 20 gg size at 100 gg/mL in sterile 4 mM HCl. Reconstitute all the other sizes at 500 gg/mL in sterile 4 mM HCl. Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from date of receipt, -20 to -70 °C as supplied. • 1 month, 2 to 8 °C under sterile conditions after reconstitution. • 3 months, -20 to -70 °C under sterile conditions after reconstitution. DATA Recombinant Human Activin A (CHO derived) Protein SDS-PAGE. 2 gg/lane of Recombinant Human Activin A (CHO derived) Protein (Catalog # 11348-AC) was resolved with SDS-PAGE under reducing (R) and nonreducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 13 and 24 kDa respectively.

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Recombinant Human Activin A (CHO derived) Catalog Number: 11348-AC BACKGROUND Activin and Inhibin are members of the TGF-β superfamily of cytokines and are involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis (1-7). Activin and Inhibin are produced as precursor proteins. Their amino terminal propeptides are proteolytically cleaved and facilitate formation of disulfide-linked dimers of the bioactive proteins (8, 9). Activins are nonglycosylated homodimers...

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