Recombinant Human IgG1 Fc

Product catalog summary

Product Description: The document describes a recombinant human IgG1 protein derived from a mouse myeloma cell line, NS0. The protein sequence spans from Pro100 to Lys330 with specific amino acid substitutions: Leu117Ala, Leu118Glu, Gly120Ala, and Thr276Ala. The protein is structured as a dimer with a predicted molecular mass of 26.6 kDa.

Specifications: The protein shows a molecular weight of 30-35 kDa under reducing conditions in SDS-PAGE. It has an endotoxin level of less than 0.10 EU per 1 μg, determined by the LAL method. The purity is greater than 95%, confirmed by SDS-PAGE with Silver Staining and Coomassie® Blue Staining. The formulation is lyophilized from a 0.2 μm filtered PBS solution.

Preparation and Storage: For reconstitution, it is recommended to use sterile PBS with at least 0.1% human or bovine serum albumin to achieve a stock solution of no less than 100 μg/mL. The product is shipped at ambient temperature and should be stored immediately upon receipt. Stability is maintained for 12 months at -20 to -70 °C as supplied, 1 month at 2 to 8 °C after reconstitution, and 3 months at -20 to -70 °C after reconstitution. It is advised to use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Catalog excerpts

Recombinant Human IgGi FcCatalog Number: 110-HGDESCRIPTION Source Mouse myeloma cell line, NSO-derived human IgG1 protein N-terminus N-terminal Sequence Ile Analysis Structure / Form Dimer Predicted Molecular 26.6 kDa Mass SPECIFICATIONS IEGRMD SDS-PAGE 30-35 kDa, reducing conditions Activity Bioassay data are not available. Endotoxin Level <0.10 EU per 1 gg of the protein by the LAL method. Purity >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. Formulation Lyophilized from a 0.2 gm filtered solution in PBS. See Certificate of Analysis for details. PREPARATION AND STORAGE Reconstitution It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 gg/mL. Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from date of receipt, -20 to -70 °C as supplied. • 1 month, 2 to 8 °C under sterile conditions after reconstitution. • 3 months, -20 to -70 °C under sterile conditions after reconstitution. of 1 Global | bio-techne.com [email protected] [email protected] USA | TEL: 800.343.7475 Canada | TEL: 855.668.8722 Europe | Middle East China | [email protected] TEL: 400.821.3475

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