Metabolic Syndrome Arrays Simultaneous determination of multiple analytes related to metabolic syndrome

Metabolic Syndrome in Clinical Research What is Metabolic Syndrome? Metabolic syndrome (MetS) is a group of cardiovascular risk factors (i.e. diabetes and prediabetes, abdominal obesity, high cholesterol and high blood pressure). It is highly prevalent, with approximately 20-25% of adults affected. It is estimated that having metabolic syndrome results in a person being three times more likely to have a stroke or heart attack, and five times more likely to develop diabetes. Underlying risk factors for MetS include: abdominal obesity, insulin resistance, physical inactivity, aging and hormonal...

Metabolic Syndrome pathways Endothelial dysfunction Vascular inflammation (atherogenesis) Astherogenic dyslipidaemia Pro-thrombotic state Pro-inflammatory state Insulin resistance Vascular dysfunction Adipose tissue Adiponectin TNFα IL-6 Resistin Adipose tissue secretes cytokines, chemokines and hormones, which are collectively known as adipokines and can link obesity with cardiovascular disorders. Obesity-related disorders such as MetS, diabetes, atherosclerosis, hypertension and coronary artery disease are linked with dysregulated adipokine expression.

Metabolic Syndrome Array 1 Metabolic Syndrome Array I contains the following assays • Interleukin-6 - pro-inflammatory adipokine with pleiotropic effects on a variety of tissues. Il-6 has been associated with obesity and features of the metabolic syndrome such as insulin resistance.2 • Tumour Necrosis Factor α (TNFα) - an inflammatory adipokine, which stimulates production of leptin in addition to IL-6 and other inflammatory mediators.3-6 • Ferritin - Iron-binding protein, the concentration in blood reflects the amount of iron stored within the body. Increased ferritin levels have been associated...

Metabolic Syndrome Array II Metabolic Syndrome Array II contains the following assays • Adiponectin - Hormone produced by adipose tissue. Adiponectin regulates lipid and glucose metabolism, low levels are associated with type 2 diabetes, which could contribute to peripheral insulin resistance in this condition.15 Cystatin C – This protein is not simply regarded as a marker of impaired renal function. It has been also related to coronary artery disease, congestive heart failure, carotid atherosclerosis and peripheral vascular disease.19 • C-reactive Protein (CRP) Acute-phase protein. Circulating...

Benefits of Metabolic Syndrome Arrays Sample Volume Comparison - Biochip Array Technology vs Standard ELISA Tests Total Number of Multi-analytical profiling using Single analyte Different Analytes Metabolic Syndrome Arrays I and II Biochip Array Technology Total Volume Test Details Test Kits Test Format Individual ELISAs Approximate average sample volume for ELISA analysis was estimated at 100 μl Benefits of the Metabolic Syndrome Arrays C • an be used in the investigation of insulin resistance, pro-thrombotic state, abnormal body fat distribution, pro-inflammatory state and atherogenic dyslipidaemia...

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1. Hu, G. et al. for the DECODE study group. Prevalence of the metabolic syndrome and its relation to all-cause and cardiovascular mortality in nondiabetic European men and women. Arch. Intern. Med. 2004, 164: 1066-1076. 2. Qatanani, M. and Lazar M.A. Mechanisms of obesity-associated insulin resistance: many choices on the menu. Genes Dev. 2007, 21: 1443-1455. 3. Stephens, J.M. and Pekala P.H. Transcriptional repression of the C/EBP-alpha and GLUT4 genes in 3T3-L1 adipocytes by tumor necrosis factor-alpha: regulation is coordinate and independent of protein systhesis. J. Biol. Chem. 1992, 267:...